ddPCR Flashcards
1
Q
ddPCR stands for?
A
Digital droplet PCR
2
Q
Why are oil droplets used for ddPCR?
A
Oil droplets form a partition that separate the template DNA functioning as individual test tubes/wells in a plate
3
Q
Where does PCR amplification happen in ddPCR?
A
within each droplet
4
Q
What kind of amplification is used for ddPCR?
A
Absolute quantification of target DNA copies, no need for standard curve
