Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Biology > ELISA > Flashcards

ELISA Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

ELISA stands for?

A

Enzyme-linked immunosorbent assay

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What kind of ELISA do we use?

A

sandwich

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

ELISA 1st step

A

Capture antibodies are coated on plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

ELISA 2nd step

A

Target Antigen (our sample, AAV serotype) attaches to antibody

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

ELISA 3rd step

A

Detection antibody carrying Horse Radish Peroxidase (or alkaline phosphatase)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

ELISA 4th step

A

Substrate (TMB or ABTS)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

ELISA 5th step

A

HRP catalyzes substrate which yields a colored product

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the purpose of ELISA?

A

protein quantification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly

Biology (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions