q1

Question 1

The practice of mating or breeding selected organisms with desirable traits

Answer 1

Classical Breeding

Question 2

Cannot control what genes are passed

Answer 2

Classical Breeding

Question 3

Steps in Classical Breeding

Answer 3

• Determine which traits are significant enough to be chosen.
• Select parents that exemplify these traits.
• Choose the best offspring from parents to produce the next generation.
• Repeat the process.

Question 4

3 Classical Breeding Selection

Answer 4

• Selective Breeding
• Hybridization
• Inbreeding

Question 5

When animals with desired characteristics are mated to produce offspring with those desired traits

Answer 5

Selective Breeding

Question 6

Two individuals with unlike characteristics are crossed to produce the best in both organisms

Answer 6

Hybridization

Question 7

• Breeding of organism that genetically similar to maintain desired traits.
• Keeps each breed unique from others

Answer 7

Inbreeding

Question 8

difference between individuals of a species

Answer 8

Variation

Question 9

Inbreeding decreases

Answer 9

Variation

Question 10

The artificial manipulation, modification, and recombination of DNA and other nucleic acid molecules to modify an organism or population of organisms.

Answer 10

Genetic Engineering

Question 11

When DNA is combined from at least two organisms

Answer 11

Recombinant DNA Technology

Question 12

DNA is cut of one organism and put into another organism

Answer 12

Gene splicing

Question 13

How are genes cut for gene splicing?

Answer 13

A bacterial plasmid is used

Question 14

Circular DNA in a bacterial cell. Very simple and easy to manipulate.

Answer 14

Plasmid

Question 15

DNA Meaning

Answer 15

Deoxyribonucleic acid

Question 16

Identify what type of Classical Breeding: Egg-Laying-Hen

Answer 16

Selective Breeding

Question 17

Identify what type of Classical Breeding: Angus cows

Answer 17

Selective Breeding

Question 18

Identify what type of Classical Breeding: Liger

Answer 18

Hybridization

Question 19

Identify what type of Classical Breeding: Burbank Potato

Answer 19

Hybridization

Question 20

Identify what type of Classical Breeding: Grapple

Answer 20

Hybridization

Question 21

Identify what type of Classical Breeding: Doberman

Answer 21

Inbreeding

Question 22

cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end

Answer 22

Restriction Enzymes

Question 23

an enzyme that can catalyze the joining (ligation) of two molecules by forming a new chemical bond

Answer 23

DNA ligase

Question 24

CRISPR

Answer 24

Clustered Regularly Interspaced Short Palindromic Repeats

Question 25

binds to the DNA and cuts it, shutting the targeted gene off

Answer 25

CAS9

Question 26

Ways in which plasmids may be introduced into host organisms

Answer 26

- Biolistic - Heat Shock Treatment - Electroporation

Question 27

A gene gun is used to fire DNA-coated pellets on plant tissues.

Answer 27

Biolistics

Question 28

Calcium Chloride Method for Bacteria

Answer 28

Heat Shock Treatment

Question 29

What temperature in Heat Shock

Answer 29

42°C for 1 min and 4°C for 2 mins

Question 30

Similar methodology as heat shock but the expansion of the membrane pores is done through an Electric shock

Answer 30

Electroporation

Question 31

A gene from one organism is transferred to a different organism.

Answer 31

Transformation

Question 32

The organisms that have DNA transferred to them

Answer 32

Transgenic Organism

Question 33

Applications of Recombinant DNA Technology

Answer 33

- Production of Gene Products - Pharmaceutical Industry and Medicine - Agriculture and Genetically Modified Organisms + Pharmaceutical Products, Manipulates DNA of singled somatic cells - Medical Applications

Question 34

Methods to Screen Recombinant Cells

Answer 34

- Selection of Plasmid DNA containing cells - Selection of trasnformed cells with desire gene - PCR detection of Plasmid DNA