PT and APTT Flashcards
What are the materials needed for the Prothrombin Time (PT) test?
PT reagent
Sample (plasma)
Test cuvette
Pipette (25 µL and 50 µL)
Incubator set at 37°C
What is the first step in the PT test procedure?
Incubate PT reagent at 37°C for at least 10 minutes.
How much sample is needed for the PT test, and how is it prepared?
Pipette 25 µL of sample into a test cuvette.
Incubate at 37°C for 1 minute.
What should be done to the PT reagent before use?
Swirl the reagent shortly before use.
How much PT reagent is added to the sample, and what is done afterward?
Add 50 µL of PT reagent.
Test the specimen and record clotting time.
What is the normal value (NV) for the PT test?
11–18 seconds
What materials are needed for the Activated Partial Thromboplastin Time (APTT) test?
Calcium chloride (CaCl₂) solution
APTT reagent
Sample (plasma)
Test cuvette
Pipette (25 µL)
Incubator set at 37°C
What is the first step in the APTT test procedure?
Prewarm CaCl₂ at 37°C for at least 10 minutes.
How much sample is needed for the APTT test, and how is it prepared?
Pipette 25 µL of sample into a test cuvette.
Incubate at 37°C for 1–2 minutes.
How much APTT reagent is added, and what happens next?
Add 25 µL of APTT reagent.
Incubate at 37°C for 3 minutes.
What is the final step of the APTT test procedure?
Add 25 µL of CaCl₂.
Test the sample and record clotting time.
What is the normal value (NV) for the APTT test?
27–42 seconds
