Protozoology Flashcards
Trypanosoma spp. having undulating membrane?
- T. brucei brucei, T. congolense.
- T. vivax- inconspicuous undulating membrane.
Stages of Trypanosoma spp. having undulating membrane:
Trypomastigote, Epimastigote.
Which trypanosome stage is IC?
Amastigote.
Which has flagellum, but ø undulating membrane?
Promastigote.
Which does ø have flagellum?
Amastigote.
Which phenotype of trypanosomes has kinetoplast ant. next to nucleus +also provided with short undulating membrane?
Epimastigote.
Which cells do Trypanosomes invade?
WBCs, macrophages.
Trypanosoma found in old + new world?
T. vivax.
For how long does Trypanosoma have?
Many years.
Salivaria vs. Stercocaira; which occur in South America?
Both.
What is the size range of trypanosomes?
8 - 39 µm.
Name of cutaneous inflammation after an infection/ infiltration with Trypanosoma.
Chancre.
Nagana caused by:
Trypanosoma vivax, T. congolense, T.brucei.
Spp ø effected by Nagana:
Wild animals + certain breeds of cattle.
Which is the largest among causative agents of Nagana?
T. brucei brucei.
Which region of Africa does the acute, more rapid form of sleeping sickness ( T. rhodensiense) occur?
Eastern.
Trypanosoma evansi causes:
Mal de caderas/ Surra.
Surra is found where in the world ?
North Africa, Asia, Central + South America.
The vector of T. brucei evansi plays a role as:
Mechanical vector.
Mal de Caderas symptoms:
Oedema of limbs.
Which animal has asymptomatic T. brucei evansi infection:
Cattle, H2O Sus.
Trypanosoma cruzi causes:
Chagas disease.
The amastigote of Trypanosoma cruzi occurs?
- In mesenchymal cells.
How can dogs be infected with Trypanosoma cruzi?
Eating bugs.
Which phenotype is ø characteristic of Trypanosoma cruzi?
Promastigote.
What is ø caused by Trypanosoma cruzi?
Megalomania.
Which has the biggest kinetoplast/ soma from 4 Trypanosoma?
T. cruzi.
How can the kissing bugs inoculate Trypanosoma cruzi in a host?
With their faeces.
Trypanosoma brucei equiperdum causes:
Dourine.
Which trypanosoma sp. causes asymptomatic infection in dogs?
T. equiperdum.
Which sp. does causative agent of Dourine belong to?
T. brucei.
In scientific terms, what is a dollar spot?
Uritricia.
Which ocular problem does Dourine ø cause?
Nystagmus, lachrymation, mydriasis.
Dourine has been found where in EU in last 10 yrs?
Italy.
What is the main sign seen towards the end of Dourine?
- Paralysis, mainly in HL.
- Genitals, skin, n.
Which of the following is detected by Giemsa staining of blood smear?
Theileria, Trypanosoma.
What type of biological vector does Salivaria- type Trypanosoma have?
- Biological: Glossina ssp. ( Tsetse flies).
- Mechanical: Stomoxys + tabanus spp ( blood sucking flies).
Which phenotype/form is possible for the pathogenesis of Leishmania?
Amastigote.
Leishmania develops from:
Amastigote.
Incubation period of Leishmania?
Several years.
Leishmaniosis vector is:
Sandfly ( Phleobotomus).
How can host be infected by Leishmania?
Female Sandfly, Vector disease, via biting.
Leishmania transmission can be:
Vector borne, Sandfly.
What is/ are characteristic of pathogenesis of Leishmaniosis?
- MPS killing amastigotes.
- Hyper- globulinemia.
Symptoms of Leishmania?
- Cutaneous form- Hair loss, hyper- keratosis, desquamation, seborrhoea, small papules, ulcers, crusts, excessive long clawa.
- Visceral form- Lymph nodes spleen + liver enlarge, fever, anaemia, emaciation, m. atrophy, ataxia, somnelence, lethargy, anorexia, vomit, diarrhoea, cachexia, PU, PD, ocular signs, rhinitis, coughing, epistaxis, melena.
Cause of death in Leishmania?
Renal insufficiency/ failure ( kidney insufficiency).
Which country lacks autochthonous cases ( indigenous, native) of Leishmaniosis?
Germany.
What is the approximate size of Giardia:
- Trophozoite 11- 17 µm.
- Cyst 10- 20 µm.
How do humans get giardia?
Swimming, contaminated drinking H2O, washing food with contaminated H2O, surfaces.
How do chickens get Giardiasis?
Infection/os, Infected H2O, H20- borne infection.
How Giardia infects?
Via contaminated H2O, food, surfaces, coprophagy.
Where can we find Giardia extra- intestinally?
Bile + pancreatic ducts.
Giardia cyst location?
Environment ( infectious form).
Giardia trophozoite location?
- Host ( vegetative form).
- In intestine, bile duct, pancreatic duct.
Which Giardia duodenalis occurs in dog?
Genotypes A- D.
Difference between Trichomonas + Tritrichomonas?
- Trichomonas 4 flagella, Birds.
- Tritrichomonas 3 flagella, Cattle.
If the following are present, more susceptible to Trichomonas.
Vit A deficiency.
How do pigeons acquire Trichomonas?
- Young pigeons ( squabs)- Via feeding of regurgitated feed content.
- Pigeons- Via kissing/ from contaminated drinking H2O.
General feature of Trichomonas in pigeons?
Penguin- like posture.
birds stand upright with head high + neck straight
What is the characteristic lesion in hepatic form of pigeon Trichomonosis?
Pea- size necrosis.
What is/ are the worst consequences in Trichomonosis in Cows?
- Early abortion ( 1st trimester, before 4th mnth)- Early abortion in 6- 16th week via absorption elimination.
- Pyometra- Grey- white, odourless fluid swarming with Trichomonas.
Age when bulls are susceptible to Trichomonas spp:
> 3 years.
How does Trichomonas gallinae reach the liver in birds?
From navel.
Geographical region with Tritrichomonas problems?
Certain states of America.
How does Tritrichomonas foetus live in genital tract?
Epi- cellularly.
Which can be found in earthworms?
Histomonas meleagridis, Heterakis earthworm as a transport host.
Predisposing factor of Histomonosis:
Turkeys kept together with chickens.
asymptomatic carriers, frequently Heterakis infected
Characteristics of Histomonosis disease in birds?
- Cyanosis of head ( blueish colour).
- + weakness, dropping of wings, drowsiness, yellow diarrhoea, death within days.
Which clinical sign is ø characteristic of turkey Histomonosis?
Head cyanosis.
Which of the following is/ are characteristic of Entamoeba histolytica trophozoites?
Eccentric endosome, Phagocytised RBCs.
What causes anosmia ( loss of smell)?
Entamoeba histolytica.
Entamoeba attacks:
RBCs.
Where does the skin abscess of Entamoeba come from?
- Skin: Erythema, alopecia, hyper- keratosis ( desquamation).
Acanthamoebosis
Free- living, soil inhabitant, opportunistic amoebae.
What is chronic granulomatous amoebic encephalitis ( GAE) in humans caused by?
Acanthamoeba castellani.
How can a dog become infected with Acanthamoeba castellani?
- Contact with still H2O, through skin lesion.
- Or with distemper.
What is the form of Naegleria fowleri does ø have?
Provided with 1 flagellum.
What is the acute 1º amoebic meningoencephalitis ( PAM) in humans caused by?
Naegleria fowleri.
What is true for infection with Amoeba?
Large nucleus + cyst.
Specific for opportunist amoebas?
Big nucleus.
Detection of Klossiella:
- Sporocysts- urine : Na hydrogencarbonate, Centrifuge, Examine sediment.
- Sporoblasts- Basophil staining, bilaminar membrane projections on surface.
What belongs to Alveolata ( meaning ‘ with cavities’, grp of protists)?
Apicomplexa, Ciliophora.
Where does the merozoite develop?
Inside Schizont.
Which statement is ø true for the development of Eimeria sp.?
Schizogony occurs in gametogony.
Pre- disposing factors to Eimeria:
6
Young age, intensive ( crowded, accelerated keeping), insufficient nutrition ( lack of vit., protein in feed), litter ( deep), neglected hygienic measures, inter- current infections.
Which Eimeria occurs extra- intestinally?
- E. ninakohyakimovae in goats’ lymph nodes + gall bladder.
- E danoilovi in ducks, lesions in cerebellum.
Difference between Isospora + Eimeria spp.?
Only Eimeria has cap, micropyle + residual bodies.
Different Eimeria spp. asking for most pathogenic stage:
- E. tenella 2nd Schizont.
- E. necatrix 2nd Schizogony.
- E. brunetti 2nd Schizogony, 3rd Gametogony.
How many Eimeria spp. cause haemorrhagic lesions in small intestine of chicken?
3 ( E. necatrix + maxima, brunetti).
How many Eimeria spp. cause haemorrahgic/ fibrinous lesions in caeca?
2 ( E. tenella + brunetti).
Which Eimeria in small intestine does ø cause haemorrhages?
E. acervulina + E. mitis.
What Eimeria spp in chicken is:
- Highly pathogenic:
- Moderately pathogenic:
- Less pathogenic:
- E. tenella + necatrix.
- E maxima.
- E acervulina + mitis.
Which Eimeria causes ladder- like white bands?
E. acervulina.
Eimeria acervulina location:
Duodenum.
Which Eimeria spp is complimentary to E. acervulina?
E. mitis.