Protozoan Flashcards
Life cycle class Rhizopoda
Trophozoite>Precystic>Cystic>Metacystic stage
Soecial organs for locomotion of protozoan
Hemotogranules
True amoeba
Peripheral chromatin
Chromatoidal bars
Entamoeba
Other amoeba
No peripheral chromatin
No chromatoid bars
Endolimax/Iodamoeba
Cyst of E. Histolytica
Matue cyst with 1-4 nuclei
Cyst of E. Coli
Matue cyst with 1-8 nuclei
Smallest amoeba
Endolimax nana
Karyosme of E. Nana
Large round dot
Nuclei of cyst of E. Nana
4 (cross eyed)
Karyosome lf Iodamoeba butschlii and Iodamoeba williamsi
With cluster of achromatic granules
Cyst of I. Butschlii and I. Williamsi
Large iodine-staining glycogen vacuole
Associated with gum infection
Entamoeba gingivalis
Major causative agent PAM (Primary Amebic Meningoencephalitis)
Acquired while diving and swimming during hot weather in brackish/freshwater
Naegleria fowleri
Causes keratitis ( from contaminated contact lens care solution)
Causes GAE (Granulomatous Amebic Encephalitis)
Acanthamoeba
Possesses whip like locomotory organelle called flagella
Mastigophora (Flagellata)
Digestive tract and Genital flagellates
Enteromonas hominis
Embadomonas intestinalis
Chilomastix mesnili
Giardia lamblia
Trichomonas homins
Trichimonas vaginalis
Trichomonas tenax
Blood and tissue flagellates
Leishmania tropica
Leishmania braziliensis
Leishmania donoani
Trypanosoma rhodesiense
Trypanosoma gambiense
Trypanosoma cruzi
Cleftlike cytosome
Embadomonas intestinalis
Associated with Gay bowel syndrome
Falling leaf motility
Old man’s face/ old man w/ eyeglasses
Giardia lamblia
Lamblia intestinalis
Habitat of Trichomonas tenax
Oral cavity
Specimen for T. Tenax
Oral scrapings
Habitat of Trichomonas hominis
Intestine
Specimen for T. Hominis
Stool
Habitat of Trichomonas vaginalis
Genitalia
Specimen for T. Vaginalis
Urine, vaginal swab or discharge female), prostatic secretion (male
Causes pingpong’s disease
T. Vaginalis
Pathogenesis of T. Vaginalis in male and female
Male: carrier
1. Asymptomatic chronic urethritis
2. Epididymis, prostate and urethra
Female
1. Burning sensation
2. Feinberg-whittington medium
Include the trypanosomes and leishmaniasis
May infect the blood, lymph nodes, muscles and RES
Hemoflagellates
Leishman-Donovan body
Leishmanial form
Amastigote
Leptomonal form
Anterior to nucleus
Promastigote
Crithidial for,
Near nucleus
Epimastigote
Trypanosomal form
Posterior to nucleus
Trypomastigote
Agent of old world cutaneous leishmanias
Oriental sore
Aleppo button
Baghdad or Jericho boil
Dry lesions
Leishmania tropica
Habitat of L. Tropica
Endothelial cells
Monocytes
Vector of L. Tropica, L. Braziliensis, L. Donovani
Phlebotomis
Lutzomyia
Pathology of L. Tropica
Localized cutaneous infection
Agent of american mucocutaneous leishmaniasis
Esoundia
Bubas
Leishmania braziliensis
Habitat of L. Braziliensis
Mucocutaneous junctions (nasal septum, mouth, pharynx)
Pathology of L. Braziliensis
Wheeping lesions
Lab diagnosis of L. Braziliensis
Dr. Montenegro’s (skin test)
Visceral leishmaniasis
Kala-azar (black fever)
Dum-dum fever
Leishmania donovani
Habitat of L. Donovani
Endothelial cells of RES
Macrophages
Pathology of L. Donovani
Splenomegaly
Severe anemia
Lab diagnosis of L. Donovani
Culture: NNN: Novy-McNeal-Nichole medium
Agent of African sleeping sickness
East africa and West africa
EA: Trypanosoma rhodiense
WA: Trypanosoma gambiense
Vector of T. Rhodiense & T. Gambiense
Glossona (tsetse fly)
Inflammation/swelling of lymph nodes
Winterbottom sign
Lab diagnosis of T. Rhodesiense and T. Gambiense
- Febrile stage: blood, lymph node aspirate
- Sleeping sickness stage: CSF
Agent of american trypanosomiasis
Chagas disease
Trypanosoma cruzi
vector if T. Cruzi
Triatomid bugs
Kissing bugs
Reduviid bugs
Assasin bugs
Cone nose bugs
Triatoma rubrofasciata
Habitat of T. Cruzi
RES, cardiac muscle, CNS
Unilateral swelling of eye lids
Romañas sign
Lab diagnosis of T. Cruzi
Chang’s medium
Xenodiagnostic test
Only significant ciliate
Balantidium coli
MOT of B. Coli
Ingestion of cysts