Protozoa-intro Flashcards
How do protozoa reproduce
asexual
What are some characteristics of Sarcomastigophora
distinct nucleus
Flagella
Asexual
Direct and indirect lifecycles
What is the main disease of Sarcomastigophora
Giaridia
What are some characteristics of Giardia
Transferred by fecal
Direct lifecycle
What is the most common form of giardia
Duodenalis
What do you see in a fecal float of Giardia
Cyst
Where do you see Giardia trophozoites
Smears
What do Giardia trophozoites look like
2 nuclei
8 flagella
Adhesive disk
How many nuclei does the Giardia cyst have
4
What stage is Giardia transferred in
Ingested in cyst stage
What are the clinical signs of Giardia
Diarrhea
Majority asymptomatic
What are some characteristics of Tritichomonas
Flagella Undulating membrane axostyle pseudocysts longitudinal binary fission
Characteristics of T. Foetus
Bovine
Venerial disease
Clinical features of T. Foetus
Early or late term abortions
breeding rates decrease
asymptomatic in bulls
How to diagnose Trich
Collect organisms
Culture organisms
Characteristics of feline trich
large bowel disease
chronic diarrhea
How do you diagnose feline Trichomoniasis
Direct fecal smear
Culture
What’s the difference between Tritrich and Giardia movement
Trich is spastic
Giardia is fluidlike
Characteristics of Trypanosomes
Transmitted by blood feeding invertebrates Kietoplast Pleomorphic cytoskeleton Single nucleus
What is characteristic of trypomastigote
Elongated cell body
single flagellum
newer infections
in blood
What is characteristic of amastigote
Rounded cell body
Short flagellum
in muscles
What is the vector of Trypanosoma cruzi
Reduviid bug
How is trypanosoma cruzi transferred
Metacyclic trypomastigotes in the feces of indirect host
What organs do trypomastigotes target
spleen
liver
lymph
What happens in acute disease of trypanosomaiasis
myocarditis
lethargy
hepatomegaly
What happens in chronic infection of trypanosoma cruzi
Dilated enlarged heart
arrhythmia
lethargy
How do diagnose trypanosoma cruzi
TESA blot
ELISA
What are the treatments for Trypanosoma cruzi
none
Characteristics of Apicomplexa
Distinct nucleus subpellicular tubules Apical complex Species/organ specific monoxenous
What species does Cystoisospora infect
dogs
cats
pigs
humans
What species does Eimeria infect
birds cattle/small ruminates horses rodents pigs
How does both cystoisopora and Eimera reproduce
Asexual and sexual reproduction -sporogony -schizogony -gametogony Sporulated oocyst
What does the host injest
Sporulated oocyst
Characteristics of sporulated Cystoisospora oocysts
2 sporocysts each with 4 sporozoites
Characteristics of sporulated Eimeria oocysts
4 sporocysts each with 2 sporozoites
What happens in sporogony
oocyst replicates and releases many sporozoites
asexual in environment
What happens in schizogony
asexual inside host
what is gametogony
sexual inside of host
What are some characteristics of Emieria
Resistant oocyst
Development in ileum and colon
intracellular
What are clinical signs of Eimeria
diarrhea
Characterictics of E. bovis
easier to diagnose
Oocysts present when disease begins
Gametogony causes majority of damage
Characteristics of E. zuernii
most severe
Schizogony produces most damage
projectile bloody diarrhea
(basically like eating chipotle)
Characteristics of Cystoisospora suis
Pigs
1st disease of suckling piglets
Contaminated feces
sporozoites penetrate villous epithelium
Clinical signs of Cystoisospora suis
pasty/liquid diarrhea
no blood in feces
How to prevent Coccidiosis
reduce fecal contamination
automatic watering systems
What is the main difference between esistoisporia and Cryptospordium
Crypto can reinfect host
Sporogony is different
clinical signs of crypto
yellow, profuse, watery diarrhea
What cell type are all Protozoa
Eukaryotic
Hosts of toxoplasma gondii
Intermediate: most mammals
Definitive: cats
Transmission of Toxoplasmosis
oocysts
raw meat
transplacental
What are the three life stages of toxoplamsa
Tachyzoite: asexual division, rapid, liver, lungs, spleen
Bradyzoites: slowly dividing, heart, brain, eyes
Sexual reproduction
What is the main way cats get Toxoplasma Gondii
Eat oocyst
also have tachyzoite: congenital, and bradyzoite: IH
Where are oocysts located
feces
What stages of Toxoplasma are unique to cat
Schizogony gametogony
What varies the prepatent period of toxoplasma gondii
how the cat is infected
Clinical signs of Toxoplasma gondii
no confirmed oocyst shedding
fever, anorexia, jaundice, death
Diagnosis of toxoplasma gondii
Fecal float
What are the DH and IH of Sarcocystis
DH: carnivore
IH: herbivore
What is the lifecycle of Sarcocystis
Gametogony occurs in intestine of DH
Sporocytes released into feces
Schizogony occurs in endothelial cells of blood vessels
Ruptures and sarcocysts form in skeletal muscle
Clinical signs of Sacocystis cruzi
IH fever diarrhea abortion decreased milk yield
What is a horse to Sarcocystis neurona
Dead end host
Schizonts develop in neural cells and destroy them
What is the DH for Cytauxzoon felis
Cats
what is the IH for Cytauxzoon felis
lonestar tick
dog tick
What is the reservoir for Cytauxzoon felis
bobcats
Life cycle of cytauxzoon felis
asexual repro in salivary glands of tick
schizogony occurs in macrophages of cat
merozoites released when macrophage ruptures
enter erythrocytes develop into piroplasms
tick ingests piroplasms
How pathogenic is cytauxzoon felis
very pathogenic
What are the two forms of cytauxzoon felis
fatal and non-fatal
What is the most destructive phase of cytauxzoon felis
Schizogenous phase
block blood vessels