Proteins: Determination of Primary Structure and Expression Flashcards
What are seven types of proteins with different functions?
Enzymes, hormones, transport, storage, structural, protective, contractile
What is the difference between a simple protein and a conjugated protein?
Simple protein is compose solely of amino acids while a conjugated protein has non AA components.
What are examples of conjugated proteins?
lipoproteins, glycoproteins, phosphoproteins, hemoproteins, flavoproteins, metalloproteins
How many amino acids are required for a protein to fold?
40
What is the common number of AA in proteins?
100-1000
How many genes in humans? How many expressed?
40,000 and 10k-15k
Some proteins are expressed at high levels and others at low. Give an example of each.
High: collagen
Low: hormones
Why are hormones harder to research than collagen?
Low expression makes identification difficult. Rely on animal hormones for research
What characteristics can be used to separate proteins?
solubility, charge, size, and affinity
Technique; Match to Characteristic
Ion Exchange Chromatography
Isoelectric focusing
Electrophoresis
Charge
Technique; Match to Characteristic
Dialysis and ultracentrifugation
Gel electrophoresis
Gel filtration
Size
Technique; Match to Characteristic
2D gel electrophoresis
Charge and size
Technique; Match to Characteristic
Affinity Chromatography
Specificity
Technique; Match to Characteristic
Paper Chromatography
Reverse-Phase Chromatography
Hydrophobic Chromatography
Polarity
In affinity chromatography, the _____ attaches to the stationary phase which is the ligand. This occurs in a lot of test tubes.
target