Proteins Flashcards
What’s the basic monomer which combines to make up a polymer called a polypeptide
Amino acid
What’s the structure of an amino acid
Has a central carbon atom
- amino group (-NH2)
- carboxyl group (-COOH)
- hydrogen atom (-H)
- R group - each amino has different R group.
How do amino acids from a dipeptide.
What’s lost
Water is removed in a condensation reaction
- OH from the carboxylate group of one amino acid
- H from the amino group of another amino acid
What’s the bond between two amino acids and where
Peptide bond
Between the carbon atom of one amino acid and the nitrogen of another
What’s the primary structure
This is just the sequence of amino acids in the polypeptide chain but this will determine the rest of the protein structure
What’s the secondary structure
the polypeptide chain doesnt remain flat and straight. Hydrogen bonds form between the amino acids in the chain. This makes it automatically coil into an alpha helix or fold into a beta pleated sheet
What’s the tertiary structure
Further folding of the secondary structure. The tertiary structure is held together by bonds between the R groups of the amino acids in the protein, and so depends on what the sequence of amino acids is. There are three kinds of bonds involved:
• Hydrogen bonds, which are weak.
• Ionic bonds between R-groups with positive or negative charges, which are quite strong.
• sulphur bridges - covalent S-S bonds between two cysteine amino acids, which are strong.
So the secondary structure is due to backbone interactions and is thus largely independent of primary sequence, while tertiary structure is due to side chain interactions and thus depends on the amino acid sequence. Thus mutations changing the primary structure may not alter the secondary structure, could have a big impact on tertiary
What’s the quaternary structure
Association of several polypeptides
such as Haemoglobin, antibodies, actin in muscle
What’s the test for proteins
Add solution to be tested, add an equal volume of sodium hydroxide solution at room temp
Add a few drops of dilute copper sulfate solution and mix gently
If positive turns purple ( peptide bonds)