proteins Flashcards

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1
Q

what is the test for protein

A

add a few drops of biuret reagent to the sample you want to test
Blue to purple if present

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2
Q

what are some examples of proteins

A

enzymes
cell membrane/in cell membrane
collagen
haemoglobin
antibodies
hormones

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3
Q

what are the groups that make up an amino acid

A

amine group
r group
carboxyl group

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4
Q

what does an amine group consist of

A

1 N AND 2 H

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5
Q

What does an R group consist of

A

C and an group

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6
Q

what does a carboxyl group consist of

A

one C pne OH and one O

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7
Q

describe peptide bond formation

A

It is between the amine group of one amino acid and the carboxyl group of another where the OH group and the H group of either bond together to form a H20 molecule release. This frees up the bond between the carbon atom and a nitrogen atom where they will bothe bond together this bond is called a peptide bond

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8
Q

What is the primary structure of a protein

A

The sequence or order of amino acids in a polypeptide chain

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9
Q

What is the secondary structure of a protein

A

-hydrogen bonds form beteeen =o and -h of nearby peptide bonds
-these Hydrogen bonds pull the primary structure into an alpha helix or a beta pleated sheet

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10
Q

What is the tertiary structure of a protein.

A

-the secondary structure folds into a more complex 3D shape pulled into position by
-hydrophobic and hydrophilic interaction
-ionic bonds
-hydrogen bonds
-disulgide bridges

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11
Q

What is the quaternary structure of a protein

A

2 or more polypeptide chains bonded together

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