Proteins Flashcards

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1
Q

What monomers form proteins?

A

Amino acids

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2
Q

What are many amino acids joined together called?

A

Polypeptide

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3
Q

How many amino acids have been identified?

A

100

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4
Q

What forms an amino acid?

A

Central carbon attached to amino (NH2) - carboxyl (COOH) - hydrogen (H) - R group (variety of different chemical groups)

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5
Q

What do amino acids differ in?

A

Their R group

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6
Q

What is formed when two amino acids join?

A

Dipeptide

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7
Q

How is a dipeptide formed?

A

two amino acids join via a peptide bond in a condensation reaction

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8
Q

How is water made in a dipeptide condensation reaction?

A

OH of carboxyl group of one amino acid and with a H from the amino group of another amino acid

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9
Q

Where is the peptide bond formed?

A

Between the carbon atom of one amino acid and the nitrogen atom of another amino acid

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10
Q

How is a polypeptide formed?

A

in series of condensation reactions where many amino acid monomers are joined together in polymerisation- resulting chain is polypeptide

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11
Q

What creates the primary structure of a protein?

A

sequence of amino acids in the polypeptide chain

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12
Q

What does the primary structure determine?

A

The proteins ultimate shape and hence it’s function

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13
Q

What is a proteins shape specific to?

A

Its function

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14
Q

What is the secondary structure of a protein?

A

Hydrogen bonds form between amino acids causing chain to coil into alpha helix or fold into beta pleated sheets

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15
Q

In the secondary structure of a protein where do the hydrogen bonds form?

A

Hydrogen of NH group has overall positive charge while Oxygen of C=O group has overall negative charge. Weak attraction between oppositely charged atoms

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16
Q

What is the tertiary structure of a protein?

A

alpha helices/beta pleated sheets folded further into more complex 3D shape
Maintained by disulphide bridges, ionic bonds and hydrogen bonds

17
Q

What is the quaternary structure of a protein?

A

Proteins formed from number of individual polypeptide chains linked in various ways. May be prosthetic groups associated with molecule (haem group in haemoglobin)

18
Q

How do we test for proteins?

A

-2cm3 sample of solution in test tube
-add equal volume of sodium hydroxide at room temperature
-add few drops of dilute copper sulfate solution and mix gently
(Biurets test)

19
Q

What indicates the presence of a protein in a Biurets test?

A

blue to purple colouration

20
Q

What are the main examples of proteins?

A

hormones, enzymes, antibodies