Protein trafficking Flashcards
What do clathrin coated vesicles do?
Carry proteins from the Golgi to the cell surface or from the cell surface to endosomes.
What do COP coated vesicles do?
Transport of vesicles from ER to Golgi and within Golgi,
What are the function of COP II proteins?
COP II proteins are required for selective export of newly synthesized proteins from the endoplasmic reticulum (ER)
What are the general functions of coat vesicles?
to shape the membrane into a bud
to capture molecules for onward transport.
What are the general functions of coat vesicles?
to shape the membrane into a bud
to capture molecules for onward transport.
What is a SNARE protein?
SNAREs are an evolutionarily conserved superfamily of small proteins with SNARE motif of 60–70 amino acids incytoplasmic domain. SNARE proteins catalyze intracellular membrane fusion in eukaryotes.
What drives the directionality of nuclear protein transport?
Ran-GTPase and importins/exportins
Ran GEF is found in the nucleus and Ran GAP is found in the cytoplasm. Ran-GTP conc is highest in the nucleus and Ran-GDP is highest in the cytoplasm.
Are proteins folded or unfolded when transported into the mitochondria?
Unfolded
Are nuclear proteins folded or unfolded when transported through nuclear pores?
Folded
Between which parts of a cell is protein transport bidirectional?
Nucleus and cytosol
What is the size of a “small” protein that can diffuse through nuclear pores?
<5000 daltons
Co translational import requires what?
GTP
Post translational import requires what?
ATP
