DNA synth Flashcards
1
Q
What reaction does DNA ligase catalyse?
A
formation of phosphodiester bond between the 5’-phosphate and 3’ hydroxyl ends
2
Q
What is Chargaff’s rule?
A
the ratio of purines to pyramidines is always 1
3
Q
Around how many histones does DNA wrap itself?
A
8 histones
which DNA wraps 1.65 times
4
Q
What is a chromatosome?
A
Nuclesome + H1 histone
5
Q
What size is the chromatin fibre?
A
11nm diameter
100 kD
6
Q
What is the average length of a loop?
A
300 nm
7
Q
What happens in the elongation phase of translation?
A
aminoacyl-tRNA binds A site
peptide bond forms
mrna-trna translocates on ribosome
