Protein testing- Biuret test Flashcards
1
Q
what is a positive colour change of the biuret test
A
Light blue to lilac
2
Q
How many peptide bonds need to be present for biuret reagent to react
A
There needs to be 2+ peptide bonds for biuret reagent to work
3
Q
what two components make up the biuret reagent
A
Sodium hydroxide and copper sulfate
4
Q
what are the steps of the biuret test
A
1- place food sample in a white spotting tile
add sodium hydroxide to sample, to make it alkali
2- add copper sulfate to sample ( which is blue colour)
3- record colour change
