Chromotography Flashcards
if a substance is interacting with the stationary phase what does this mean
it is taking longer to reach the solvent front
if a substance is interacting strongly with the mobile phase what does it mean
the substance is moving quickly
what is the tlc plate made of
aluminium and is coated in silica
if a substance is more polar, what phase is it likely to interact with
more polar means it will interact with stationary phase stronger
what phase will a less polar substance interact with
the mobile phase, meaning it will rise with the solvent and be higher up the tlc plate
what does it mean if a substance has a high affinity to a stationary phase
it is strongly interacting with the tlc plate, taking longer to elute up the plate
what is the equation for rf
distance travelled by sibstance / distance travelled by solvent