Protein synthesis

Question 1

how many combinations of codons

Answer 1

64

Question 2

code written in what direction

Answer 2

5’-3’

Question 3

AUG

Answer 3

initiation codon (meth)

Question 4

UAA,UGA,UAG

Answer 4

stop codons

Question 5

which 2 AA have only one codon

Answer 5

meth and trp

Question 6

mitochondria has how many proteins that it can synthesize itself

Answer 6

13

Question 7

what sequence is added after transcription to tRNA

Answer 7

CCA on 3’

Question 8

which side is CCA added to

Answer 8

3’

Question 9

describe aminoacylation of tRNA

Answer 9

o on carboxyl side of AA attacks the alpha phosphate in ATP and is now activated, class 1 or class 2 aminoacyl-tRNA synthase can now help tRNA to bond

Question 10

how do class 1 and class 2 aminoacyl- tRNA synthases the

Answer 10

class one attacks 2’ OH whereas class 2 attacks 3’

Question 11

fMet is the first codon for peptide chains in the ____

Answer 11

mitochondria

Question 12

eIF

Answer 12

eukaryote initiation factors

Question 13

which eIF brings in tRNA (met)

Answer 13

eIF 5B attached to GTP

Question 14

where does tRNA met bind to

Answer 14

P site

Question 15

is mRNA present when tRNA (met) binds?

Answer 15

no

Question 16

what happens when mRNA comes into pre-initiation complex

Answer 16

• requires energy ATP

- gets scanned untill first AUG is found (the ones before dont get decoded

Question 17

what happens afte AUG is found

Answer 17

eIF leave and large subunit (60S) comes in forming the 80S ribosome

Question 18

which elongation factor helps form the peptide bond?

Answer 18

EF-p

Question 19

which elongation factor binds tRNA into A site?

Answer 19

EF-tu with GTP

Question 20

which elongation factor pushes dipeptide from A site to P site?

Answer 20

EF-G with GTP

Question 21

what comes in to inititate termnation

Answer 21

release factor - hydrolyzes the peptidyl link and components dissociate

Question 22

which terminal would the post translation modification of acetylation aoccur?

Answer 22

the N-terminal of an amino acid

Question 23

what are other examples of post translational modifications

Answer 23

removal of the Met or fmet
removal of some specific segments in the chain
attaching carbohydrates

Question 24

E1

Answer 24

activating (ATP)

Question 25

E2

Answer 25

conjugating

Question 26

E3

Answer 26

ligating (joining together)

Question 27

where does ubiquinone attach to?

Answer 27

lysine (only ) on the target protien

Question 28

What are E1,2,and 3

Answer 28

enzymes required to help attached ubi to target protien (requires energy ATP in E1 step)