Protein synthesis

Question 1

Role of eIF2

Answer 1

Brings trnai to the 40s ribosome subunit to the p site

Question 2

eIF1 + 1a

Answer 2

Prevent the 60s subunit associating

Question 3

eif5

Answer 3

GAP activity that hydrolyses GTP to GDp on the eif2. This causes it to dissociate

Question 4

eif3

Answer 4

binds the 40s subunit to separate the subunits

Question 5

eif6

Answer 5

binds the 60s subunit to separate the ribosome

Question 6

eif2b

Answer 6

GEF that resets the trna from GDP bound to gtp bound

Question 7

AUG in what context is needed

Answer 7

kozak sequence

Question 8

eEF1 alpha

Answer 8

brings new AminoacyltRNAs to the a site

Question 9

eEF1 beta

Answer 9

resets the GTP state acting as a GEF

Question 10

What has peptidyltransferase activity

Answer 10

28s rRNA in 60s subunit

Question 11

Describe transpeptidation reaction

Answer 11

N terminal of A site AA bound to trna performs a Nu attack on the AA of trna in p site.

Question 12

What drives ribosome translocation

Answer 12

Hydrolysis of GTP from eEF2

Question 13

what causes translational termination

Answer 13

eRF1 binds to all 4 stop codons