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GER > Patty eukaryotic gene expression lecture 4 > Flashcards

Patty eukaryotic gene expression lecture 4 Flashcards

1
Q

What is NELF

A

Negative elongation factor. Bind to the CTD at the promoter proximal pause and prevents elongation. This allows for the 5’ cap to be added

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2
Q

What is DSIF

A

DRB sensitivity inducing factor. Binds CTD and Inhibits elongation during the promoter proximal pause, allows 5’ cap to be added

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3
Q

What Phosphorylates serine 5

A

TFIIH

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4
Q

What phosphorylates serine 2

A

CTDK-1 part of the P-TEFb

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5
Q

What does P-TEFb phosphorylate

A

Serine 2 via its CTD-k1 domain as well as NELF and DSIF

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6
Q

How many NT are added before TFIIB dissociates and promoter escape occurs

A

8-9NT

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7
Q

How many NT are added before the promoter proximal pause

A

20-50NT

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8
Q

What happens when NELF is Phosphorylated

A

It dissociates from the CTD and elongation inhibition is relieved

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9
Q

WHat happens when DSIF is phosphorylated

A

It switches it activity from inhibition to activation and recruits elongation activators such as ELONGIN

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10
Q

What happens when serine 2 is phosphorylated

A

3’ processing proteins are recruited to the CTD

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11
Q

What happens when the serine 5 is phosphorylated

A

5’ Capping

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12
Q

What removes the phosphate from serine 5

A

Serine 5 phsophatase

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13
Q

What is the complex called after it as escaped the promoter

A

The early elongation complex

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14
Q

When does capping occur

A

Co transcriptionally after the serine 5 has been added

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15
Q

Which domain has the CTD

A

B’ like domain

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16
Q

What cleaves the gamma phosphate from the 1st NT in the transcript

A

Nucleotide phosphorylase

17
Q

Which 2 phosphate groups form the 5’ to 5’ linkage in the capping reaction

A

Beta phosphate in transcript with the alpha phosphate in GTP

18
Q

What enzyme adds Methyl group to Guanine cap

A

Guanylyl-N7-methyltransferase

19
Q

What adds methyl groups to OH groups in other transcript nucleotides?

A

Nucleotide-2-O-methyltransferase adds me to 2’ OH in the sugars of other NTs

20
Q

What is the Methyl donor for the capping reaction

A

SAM s-adenosyl-methionine

GER (6 decks)

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