protein synthesis Flashcards

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1
Q

what is a genome

A

complete set of genes in a cell

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2
Q

what is a proteome

A

complete set of proteins the cell can produce

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3
Q

what is mRNA

A

copy of a gene from dna.It has 3 bases in the sequence that code for an amino acid,codon
linear structure

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4
Q

where is mRNA created and why

A

created in the nucleus then leaves through nuclear pore to carry genetic code of one gene to ribosome this is because dna is too large to fit through the nuclear pore and would be at risk of being destroyed in the cytoplasam by enzymes

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5
Q

what is the function of RNA

A

to copy and transfer the genetic code from dna to a ribosome

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6
Q

what is Rrna

A

combines with a protein to make a ribosome

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7
Q

describe structure of Trna

A

-single stranded ,folded into clover shape as H bonds hold together complementary bases
-amino acids bind to specific bind to a specific region of the molecule
-the anticodon found on the trna molecule is complementary to a specific codon on mrna

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8
Q

function of trna

A

to attach to 1 of 20 amino acids and transfer this amino acid to the ribosome to create a polypeptide chain

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9
Q

what are the 2 stages of protein synthesis

A

transcription and translation

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10
Q

what is transcription

A

one gene on dna is copied onto mRNA

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11
Q

describe steps of transcription

A

.DNA strands are separated by breaking hydrogen bonds​ with dna helicase
.Transcription is the synthesis of an mRNA copy of a gene;​
.The copy is complementary to the DNA template/antisense strand;​
.RNA polymerase attaches to DNA​
.RNA nucleotides matched to exposed complementary bases;​
.Adjacent nucleotides are joined by a phosphodiester bond together to make a strand of mRNA;​
.mRNA molecule separates from DNA​

In eukaryotes only: mRNA is spliced to remove introns​

.Mature mRNA leaves the nucleus through nuclear pores

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12
Q

what is translation

A

where mrna joins with a ribsome and corresponding trna molecules bring specific amino acid the codon codes for

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13
Q

describe steps of translation

A

mRNA attaches to the ribosome at a start codon​

tRNA with a complementary anticodon attaches to the mRNA codon​

This tRNA is attached to a specific amino acid​

Amino Acids are joined by a peptide bond​

Using energy from ATP​

tRNA is released from the ribosome and a new on enters​

Ribosome moves along the mRNA to synthesise a complete polypeptide

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