protein synthesis Flashcards
what is a genome
complete set of genes in a cell
what is a proteome
complete set of proteins the cell can produce
what is mRNA
copy of a gene from dna.It has 3 bases in the sequence that code for an amino acid,codon
linear structure
where is mRNA created and why
created in the nucleus then leaves through nuclear pore to carry genetic code of one gene to ribosome this is because dna is too large to fit through the nuclear pore and would be at risk of being destroyed in the cytoplasam by enzymes
what is the function of RNA
to copy and transfer the genetic code from dna to a ribosome
what is Rrna
combines with a protein to make a ribosome
describe structure of Trna
-single stranded ,folded into clover shape as H bonds hold together complementary bases
-amino acids bind to specific bind to a specific region of the molecule
-the anticodon found on the trna molecule is complementary to a specific codon on mrna
function of trna
to attach to 1 of 20 amino acids and transfer this amino acid to the ribosome to create a polypeptide chain
what are the 2 stages of protein synthesis
transcription and translation
what is transcription
one gene on dna is copied onto mRNA
describe steps of transcription
.DNA strands are separated by breaking hydrogen bonds with dna helicase
.Transcription is the synthesis of an mRNA copy of a gene;
.The copy is complementary to the DNA template/antisense strand;
.RNA polymerase attaches to DNA
.RNA nucleotides matched to exposed complementary bases;
.Adjacent nucleotides are joined by a phosphodiester bond together to make a strand of mRNA;
.mRNA molecule separates from DNA
In eukaryotes only: mRNA is spliced to remove introns
.Mature mRNA leaves the nucleus through nuclear pores
what is translation
where mrna joins with a ribsome and corresponding trna molecules bring specific amino acid the codon codes for
describe steps of translation
mRNA attaches to the ribosome at a start codon
tRNA with a complementary anticodon attaches to the mRNA codon
This tRNA is attached to a specific amino acid
Amino Acids are joined by a peptide bond
Using energy from ATP
tRNA is released from the ribosome and a new on enters
Ribosome moves along the mRNA to synthesise a complete polypeptide