Protein Catabolism Chart Flashcards
What does S I M mean?
Sulfur
Indole
Motility
What medium is the SIM Deep test performed on?
A Basic T-soy semisolid agar
What is the indicator of any H2S production in the SIM Deep test?
Ferrous sulfate
What are the three things the SIM Deep test screens for?
For the ability of an organism to:
(1) BREAKDOWN the AA cysteine found in peptone or REDUCE thiosulfate in anaerobic respiration
(2) BREAKDOWN the AA tryptophan and PRODUCE indole and pyruvic acid
(3) MOVE in the medium
In a SIM Deep test what does a black precipitate in the agar indicate?
That H2S has been produced due to cysteine digestion
In a SIM Deep test after the addition of kovac’s reagent to the top of the agar what does a red ring indicate?
The production of indole and a (+) test
In a SIM Deep test what does a feathering away or cloudiness from the stab line indicate?
A (+) test for motility
In the SIM Deep test what are two enzymes found in a positive test?
(1) Cysteine desulfurase
(2) Thiosulfate reductase
In a SIM Deep test the organisms that are able to digest tryptophan have what enzyme?
Tryptophanase
True or false:
A (+) motility SIM Deep test does not indicate that the bacteria possess flagella for movement.
False
What medium is the Urea slant test performed on?
Urea agar
What is the pH indicator for a Urea slant Test?
Phenol Red
What is the Urea Slant test screening for?
To determine if the organism is able to BREAKDOWN urea.
In a Urea Slat test a color change to a bright pink-fuchsia indicates what?
A (+) test
In a Urea slant test how do we know if bacterial activity is rapid or delayed?
If the color is THROUGHOUT the medium the activity is RAPID. If the color is only on the SLANT the activity is DELAYED.
In a Urea Slant test what does it mean if the agar remains yellow?
A (-) test
In a Urea Slant test a positive result indicates the presence of what enzyme?
Urease
In a Urea Slant test the organism uses urease to do what?
Breakdown urea to ammonia
In a Urea Slant test the breakdown of urea to ammonia causes what?
A rise in pH of ~8.4 or more.
Note: a pH of ~6.8 or less does not cause color change
What medium is the Phenylalanine slant test performed on?
Phenylalanine agar
What is the Phenylalanine slant test screening for?
Used to determine if an organism has the ability to deaminate or break off the amino group from the phenylalanine amino acid.
In a Phenylalanine slant test after adding 10% ferric chloride to the top of a slant what color will a (+) result turn?
Green
In a Phenylalanine slant test after adding 10% ferric chloride to the top of a slant what color will a (-) result turn?
Yellow
In a Phenylalanine slant test a (+) result indicates the presence of what enzyme?
Phenylalanine deaminase
