Gram Positive Test Chart Flashcards
In a coagulase test what is the composition?
Rabbit plasma
What is a coagulase test screening for?
The presence of coagulase enzyme
What are the possible results for a coagulase test?
Positive= White flakes Negative= None
What does the enzyme coagulase do?
Allows bacteria to form a “mask” against phagocytosis
What is the composition of the DNAse test?
DNA methyl green dye
What is the DNAse test screening for?
Presence of DNAse enzyme
What are the possible results for the DNAse test?
Clearing of media around bacterial growth
What does the enzyme DNAse do to achieve a positive result?
Digest the DNA
What is the composition of Novobiocin?
Novobiocin in a disc
What is the Novobiocin test screening for?
Sensitivity or resistance
What are the possible results of a Novobiocin test?
If zone of clearing is more that 16mm= organism is sensitive to drug will grow around it.
If zone of clearing is less than 16mm= organism is resistant to the drug will grow right through it.
What is the composition of a catalase test?
Hydrogen peroxide (H2O2)
What does the catalase test screen for?
The presence of the enzyme catalase
What are the possible results of the catalase test?
Bubbles of O2
What do the bubbles indicate in the catalase test?
The presence of the enzyme catalase
What does the catalase test differentiate between?
Staph and Strep organisms
In the Bacitracin test what is the composition?
Bacitracin in a disc
What is the Bacitricin test screening for?
Sensitive/resistance
What are the possible results of the bacitricin test?
If the zone of clearing is more than 10mm= organism is sensitive to the drug will grow around it.
If the zone of clearing is less than 10mm= organism is resistant to the drug will grow right through it.
What is the bacitracin test used for?
To distinguis between b-hemolyzers strep. pyogens, and strep. agalactiae
What is the composition of optochin?
Optochin in a disc
What does the optochin test screen for?
Sensitive/resistance
What are the possible results of the optochin test?
If the zone of clearing is more that 14mm the organism is sensitive to the drug and will grow around it
If the zone of clearing is less than 14mm the organism is resistant to the drug and will grow through it.
What is the optochin test used for?
To distinguish between a-hemolyzers strep. mitis and strep pneumoniae
