Proteases Flashcards
Degree of hydrolysis
(peptide bonds cleaved/total number of peptide bonds) * 100
Endoproteases hydrolyze
peptide bonds in the interior of the polypeptide chain
Exoproteases act on the
N-terminal (amino) or the C-terminal (carboxy) of the polypeptide chain
*Trypsin hydrolyzes peptide bonds only
at lysyl and argininyl residue at the carboxyl side
Chymotrypsin hydrolzyes peptide bonds on the
the carboxyl side of aromatic/large hydrophobic amino acids
Milk turns into a gel structure through
the action of coagulating enzymes
Milk protein consists mostly of
Casein (80%)
K-casein
+ aggregate to form micelles
+ is hydrophilic (other hydrophobic)
Glycomacropeptides (peptide + sugar) on the surface of casein keep
the micelles soluble in milk