Enzyme Kinetics

Question 1

Kinetics

Answer 1

Study of the rates of reactions

Question 2

Enzyme Kinetics

Answer 2

Study of rates of enzyme-catalyzed reactions

Question 3

k = rate constant

Answer 3

Rate is directly related to [A] by a proportionality constant

Question 4

Rate (V) =

Answer 4

• (/\A / /\ T) = /\ P / /\ T

Question 5

Rate is directly related to [A] by a proportionality constant

Answer 5

k = the rate constant

Question 6

Overtime concentration of reactant will _____ and the product will _____

Answer 6

decrease; increase

Question 7

First-Order Reaction: A–> P

Answer 7

V = k[A]

The rate is directly proportional linear to the reactant concentration (s-1)

Question 8

Second-order reaction: A+B –> P

Answer 8

V = k[A][B]

the rate is proportional to the concentration of each reactant (M-1s-1)

Question 9

Rate of catalysis V0

Answer 9

Number of moles of product formed per second when the reaction is just beginning

Question 10

Two variables for the effect of the use of catalysis

Answer 10

Concentration of Substrate and Rate

Question 11

The kinetic characteristics of many enzymes can be explained by

Answer 11

Michaelis-Menten Equation

Question 12

Vmax

Answer 12

the rate of the reaction when there are enough substrate molecules to completely fill (saturate) the enzyme’s active sites

Question 13

Vmax shows how

Answer 13

fast can an enzyme catalyze the reaction

Question 14

Michaelis Constant (KM)

Answer 14

the substrate concentration at which half the enzyme’s active sites are occupied by substrate

Question 15

KM is a measure of

Answer 15

the affinity of the enzyme for its substrate

Question 16

Low KM

Answer 16

high affinity
(less substrate is required to fill the enzyme’s active sites)

Question 17

High KM

Answer 17

low affinity

(more substrate is required to fill the enzyme’s active sites)