Properties of Enzymes Flashcards
What effect do enzymes have on a reaction?
They increase the rate by decreasing activation energy. (Have no effect on thermodynamic equilibrium)
Explain the induced-fit mechanism
Where the approach of the substrate changes the enzyme’s active site so that the substrate can bind effectively
What does the Michaelis constant (Km) measure?
The affinity of the enzyme for its substrate
What are isozymes?
They are a group of enzymes that catalyse the same reaction
What is chymotrypsin?
It is a serine protease produced by the pancreas
What amino acids make up the catalytic triad in chymotrypsin?
Serine, aspartate, and histidine
What are two other serine proteases?
Trypsin and elastase
What are the five basic strategies used by enzymes to form and stabilise the transition state?
The use of binding energy Covalent catalysis General acid-base catalysis Metal ion catalysis Catalysis by approximation
