Prokaryotic diversity Flashcards
Distinguish between classification and identification
Identification is matching characteristics of an unknown organism to lists of known organisms, and classification is placing organisms in groups of related species.
Describe how to make and use a dichotomous key
Identification key based on successive yes or no questions, used to distinguish between physical and functional characteristics.
List the characteristics of proteobacteria
Gram-negative, chemoheterotrophic, five classes (alphaproteobacteria, betaproteobacteria, gammaproteobacteria, deltaproteobacteria, epsilonproteobacteria)
List the characteristics shared by alphaproteobacteria
They are oligotrophs (can grow with very low levels of nutrients), many have stalks or buds (prosthecae), a few human pathogens.
Alphaproteobacteria-One of the most abundant microorganisms in the ocean, extremely small, rod or crescent-shaped, nonmotile, advantage in low-nutrient environments, important role in Earth’s carbon cycle
Pelagibacter
Alphaproteobacteria-Motile bacillus, fix nitrogen in the roots of leguminous plants
Rhizobium and Bradyrhizobium
Alphaproteobacteria- Motile bacillus, Plant pathogen, causes crown gall disease, inserts plasmid into plant cells, inducing a tumor
Agrobacterium
Alphaproteobacteria- Non motile pleomorphic obligate intracellular parasite, causes spotted fevers…R. prowazekii, R typhi, R, richettsii (Rocky mountain spotted fever), transmitted by insect and tick bites
Rickettsia
Alphaproteobacteria- Nonmotile obligate intracellular parasite, coccoid or ellipsoid, transmitted by ticks, cause ehrlichiosis
Ehrlichia
Alphaproteobacteria- Motile, pleomorphic, human pathogen, B. henselae (cat-scratch disease)
Bartonella
Alphaproteobacteria- Motile, coccobacillus, obligate parasite of mammals, survives phagocytosis, causes brucellosis (usually due to consumption of unpasteurized dairy)
Brucella
List the characteristics shared by betaproteobacteria
They are eutrophs (require copious amounts of organic nutrients), aerobes and anaerobes, Include many human pathogens (some life-threatening)
Betaproteobacteria-Motile bacillus, B. cepacia (degrades more than 100 organic molecules; can grow in disinfectants), B. pseudomallei (causes melioidosis)
Burkholderia
Betaproteobacteria- Non motile rods, B. pertussis (whooping cough)
Bordatella
Betaproteobacteria- Non motile, flattened diplococci (coffee beans), obligate aerobes, N. gonorrhoeae (gonnorrhea), N. meningitidis (meningococcal meningitis)
Neisseria
List the characterisitics shared by gammaproteobacteria
Most diverse class of gram-negative bacteria, aerobes and anaerobes, chemoautotrophs, photoautotrophs, heterotrophs, include important environmental microbes and many human pathogens
Gammaproteobacteria-Grows in aquatic sediments; uses gliding motility;can be problematic for sewage treatment, chemoautotrophic; oxidize H2S for energy
Beggiatoa
Gammaproteobacteria- Non motile coccobacillus, F. tularensis (tularemia)
Francissela
Gammaproteobacteria- Pseudomonadales; motile bacillus opportunistic pathogens; nosocomial infections, commonly found in soil, P. aeruginosa (wound and urinary tract infection)
Pseudomonas
Gammaproteobacteria-Pseudomonodales; Non motile bacillus, A. baumanii (opportunisitic nosocomial respiratory pathogen, resistant to antibiotics)
Acinetobacter
Gammaproteobacteria-Legionellales; Motile bacillus, found in streams/warm-water pipes/cooling towers, L. pneumophila (legionellosis/Legionnaires disease)
Legionella
Gammaproteobacteria-Legionellales; Non motile bacillus, C. burnetti (Q fever, transmitted via aerosols or milk, obligate intracellular pathogen)
Coxiella
Gammaproteobacteria-Legionellales; Motile curved rods, found in aquatic habitats, V. cholera (cholera), V. parahaemolyticus (gastroenteritis), V. vulnificus (life threatening cellulitis and blood infections), Aliivibrio fischeri (not human pathogen, can cause fluorescence, aquatic animals)
Vibrio
Gammapreoteobacteria- Enterobacteriales characteristics
Enterics (inhabit the intestinal tract), facultative anareobes that ferment carbohydrates
Gammaproteobacteria- Enterobacteriales; Motile coliform (ferments lactose completely), E. coli (fecal contamination, foodborne illness, uti)
Escherichia
Gammaproteobacteria- Enterobacteriales; Motile non coliform, common foodborne illness, S. typhi (typhoid fever)
Salmonella
Gammaproteobacteria- Enterobacteriales; Nonmotile coliform, causes bacillary dysentery
Shigella
Gammaproteobacteria- Enterobacteriales; Nonmotile coliform, K. pneumoniae (pneumonia)
Klebsiella
Gammaproteobacteria- Enterobacteriales; Motile coliform, produces red pigment, common cause of nosocomial infections, commonly found in showers and bathrooms (moist environments)
Serratia
Gammaproteobacteria- Enterobacteriales; non coliform, swarming motility, colonies form concentric rings
Proteus
Gammaproteobacteria- Enterobacteriales; Non motile coliform, Y. pestis (plague), transmitted via fleas
Yersinia
Gammaproteobacteria- Enterobacteriales; Motile coliform, E. cloacae/ E. aerogenes (cause uti and nosocomial infections)
Enterobacter
Gammaproteobacteria- Enterobacteriales; Motile noncoliform, plant pathogens
Erwinia
Gammaproteobacteria- Pasteurellales; Non motile pleomorphic pathogen of domestic animals, P. multocida (transmitted to humans via animal bites)
Pasteurella
Gammaproteobacteria- Pasteurellales; Non motile coccobacillus, requires X( heme) and V (NAD) factors, H. influenza (meningitits, earaches, epiglottitis)
Haemophilus
Distinguish between coliform and non coliform. Give examples for each.
Coliform- can ferment lactose completely (Escherichia, serratia, enterobacter) and non coliform bacteria does not ferment lactose completely (Salmonella, proteus, shigella)
List the characteristics of Deltaproteobacteria
Noted sulfate reducers and microbes with predatory behavior, sulfate reducers and anaerobic and other groups are aerobes.
Deltaproteobacteria- Famously fast motile bacillus, attack other gram-negative bacteria (predatory)
Bdellovibrio
Deltaproteobacteria- Motile bacilli that use sulfur or sulfate instead of O2 as final electron acceptors; aerotolerant, found in anaerobic sediments (bottom of streams) and intestinal tracts
Desulfovibrio
Deltaproteobacteria- Myxo-mucus, coccoid bacteria, move by gliding, leave a slime trail, cells aggregate and form a fruiting body containing myxospores
Myxobacteria
List the characteristics shared by Epsilonproteobacteria
Smallest class of proteobacteria, Helical or curved, microaerophilic
Epsilonproteobacteria- One polar flagellum, C. jejuni (foodborne intestinal disease)
Campylobacter
Epsilonproteobacteria- Multiple flagella, causes peptic ulcers and stomach cancer
Helicobacter