Principles in Plant Development Flashcards
traits of plants which impact the way they develop
-high plasticity
-rigid cell walls, differential growth rather than movement is therefore the important thing
-permanently rooted, so environment impacts a lot
-no permanent germ line, so7 no sequestering of the germ line
clonal analysis
mutating a single cell, and seeing which cells are affected later on (e.g. lack of pigmentation)
corn experiment looking at cell fates
changed random bits of the embryo and tracked how much ir affected, found no real rigid fate map
how can you assess growth plasticity?
put the apex of a leaf in culture and see how much goes- can get full sets of leaves
how do leaf cells differentiate?
according to position- cells at specific layers differentiate into different cell types
what does Knotted1 do?
involved in meristem establishment and maintenance
Kn1 loss of function
no meristem
Kn1 gain of function
ectopic growths in the leaf
what does auxin do in leaf formation?
drives expression of reporter genes, allowing gradients to be established
tangled mutants
have abnormal transverse cell divisions- but not abnormal morphology, suggests some kind of supercellular control?
Kn1 range of impact
seems to be able to affect cells other than the ones directly producing it- protein moving to create gradients?
examples of how environmental cues can be perceived by plants
nod factors, proteins like flavonoids and spirolactone
principles of perception > response pathway
signal binds to a receptor molecule, proteins phorphorylated or other signalling method such as calcium signalling, activation of transcription factors, response
what might distinguish germline and somatic tissues?
markings on germline cells?
epigenetic marks and increased chromatin volume can be associated with mother cells
