pretest

Question 1

most common adhesive for paraffin sections

Answer 1

Mayer’s Egg albumin

Question 2

part of microtome that holds tissue in place

Answer 2

chuck (tissue block holder)

Question 3

type of mold that has series of interlocking plates

Answer 3

compound

Question 4

what step of tissue processing is not properly done when the tissue is soft

Answer 4

fixation

Question 5

in histopathology, what is the most important part?

Answer 5

fixation

Question 6

the embedding media should be __ greater than the tissue

Answer 6

25x

Question 7

used to filter wax to allow reuse of paraffin

Answer 7

green’s no.904

Question 8

inventor of rocking microtome

Answer 8

paldwell trefall

Question 9

used to remove “burr” and polishing

Answer 9

stropping

Question 10

what do you call the process of precise positioning of tissue in a slide

Answer 10

orientation

Question 11

toxic dehydrating agent

Answer 11

methanol/ methyl alcohol

Question 12

how much paraffin should be around your tissue after trimmiming

Answer 12

2mm

Question 13

type of aluminum hematoxylin that uses mercuric oxide

Answer 13

harris hematoxylin

Question 14

factors that enhance rate of fixation

Answer 14

size and thickness
moderate temp
agitation

Question 15

factors that retard rate of fixation

Answer 15

size and thickness
cold temp
presence of anatomical barriers (mucus, fats, blood)

Question 16

a clearing agent that is used when xylene and benzene are not available

Answer 16

toluene

Question 17

most common and the fastest decalcifying agent

Answer 17

5-10% nitric acid

Question 18

used for hard sharpening and remove nicks

Answer 18

honing

Question 19

how much of the clearing agent should be needed

Answer 19

10x