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Histotechniques > instrumentation > Flashcards

instrumentation Flashcards

1
Q

3 essential parts of a microtome

A

block holder
knife and knife carrier
pawl, ratchet feed wheel & adjustment screws

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2
Q

simplest among the microtomes

A

rocking

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3
Q

microtome used for celloidin-embedded sections

A

sliding

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4
Q

microtome invented in 1789

A

sliding by adam

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5
Q

when was freezing microtome invented and who invented it?

A

1848; queckett

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6
Q

microtome used in electron microscope

A

ultrathin

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7
Q

cryostat is used in

A

fresh tissue microtomy

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8
Q

honing-

A

hard sharpening
nicks
heel to toe

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9
Q

hone with much more coarse effect

A

fine carborundum

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10
Q

enumerate the three knives

A

plane concave- 25mm
biconcave- 120mm
plane wedge- 100 mm

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11
Q

what temp is infiltration process maintained

A

55-60

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12
Q

refractive index of a slide

A

1.518

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13
Q

drying process is maintained at 56-60 degrees for how long?

A

2 hours

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14
Q

perfect angle

A

15 degrees

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15
Q

bevel angle

A

27-32 degrees

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16
Q

clearance angle

A

5-10

17
Q

slide measurement

A

76mmx25mm

18
Q

hone for manual sharpening

A

belgium yellow

19
Q

most common type of microtome for routine

A

rotary

20
Q

most common microtome for rapid preparations

A

cryostat/cold microtome

21
Q

inventor of rocking microtome

A

padwell trefall

22
Q

most dangerous type of microtome

A

sliding

23
Q

what year was minot microtome invented

A

1885-86

24
Q

hone that gives a more polishing effect

A

arkansas

25
Q

t/f: freezing microtome is used for undecalcified and unembedded tissues in frozen state

A

f- undehydrated and unembedded

26
Q

two types of sliding microtome

A

base sledge

standard sliding

27
Q

two microtomes used for paraffin embedded tissues

A

rotary and rocking

28
Q

a type of sliding microtome with a stationary knife

A

base sledge

Histotechniques (7 decks)

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