clearing, impregnation, trimming, sectioning

Question 1

clearing agent that causes aplastic anemia

Answer 1

benzene

Question 2

tissue should be surrounded by atleast ___ of wax after trimming

Answer 2

2mm

Question 3

enumerate the ways of paraffin impregnation and embedding

Answer 3

manual processing (hand processing)
automatic processing (ex. autotechnicon)
vacuum embedding

Question 4

enumerate types of sections and their usual thickness

Answer 4

paraffin: 4-6 micra
frozen: 10 micra
celloidin: 10-15 micra

Question 5

most commonly used water soluble wax

Answer 5

carbowax

Question 6

used specifically for eye impregnation/embedding

Answer 6

bioloid

Question 7

has the fastest result among the 3 ways of paraffin impregantion

Answer 7

vacuum

Question 8

a way of paraffin impregnation that uses constant agitation

Answer 8

automatic

Question 9

t/f: automatic processing has 12 steps of processing

Answer 9

T

Question 10

enumeration: what can be used to pick section cuts

Answer 10

[FInSCa]
fat-bladed forceps
index finger
spatula
camel hairbrush

Question 11

an adhesive that increases viscosity

Answer 11

glycerine

Question 12

section adhesive used for solid tx biopsies

Answer 12

poly-l-lysine

Question 13

used for cytospin preparations of proteinaceous/ bloody material

Answer 13

3-aminopropylthriethoxysilane (APES)

Question 14

temp used in wax bath of automatic processing

Answer 14

3 degrees celsius above wax melting point