Presentations 5 and 6

Question 1

What does the acronym STAR stand for when referring to STAR elements?

Answer 1

Sensitive to Taz1 and Rif1
(B)

Question 2

In deletion analysis of star elements, boundary activity was found in how many elements with what sequence repeat?

Answer 2

5, GGTTA
(E)

Question 3

Which protein is recruited by Rif1 that plays a role in Mcm4’s inhibitory effect of helicase activity?

Answer 3

Dis2
(C)

Question 4

With the help of Rif1 and Taz1, Star elements are capable of many things. Which of these are/is not included in its function?

Question 5

In normal taz1Δ cells boundary activity is reduced, in taz1Δ cells that express Gal4 binding domain-(Rif1, Sds21, Dis2) fusion proteins and have Gal4 binding sites binding sites in place of IR-R, boundary activity is still supported. This suggests:

Question 6

The ‘GGTTA’ repeat in STAR elements is the binding site of what protein.

Answer 6

Rif1
(A)

Question 7

Rif1 recruits and interacts with Dis2 and Sds21 phosphatases that delay phosphorylation events necessary for origins firing during DNA replication. When these interactions are interrupted, boundary activity of Rif1 is reduced. This suggests:

Question 8

In wild type cells with IR-R, the silent mating type region of heterochromatin is replicated early. When STAR elements are inserted in place of IR-R, replication is delayed in the whole heterochromatic region but nearby euchromatic origins are unaffected. This result indicates:

Question 9

DNA elements that help regulate origin firing also_____ :

Question 10

What repeat has been found to correlate their boundary activity to the number of repeats?

Question 11

By measuring incorporation of BrdU in the DNA, they monitored replication over 60 kb and found that___:

Question 12

What occurs when Mcm4 replicative helicase is mutated?

Question 13

Which CAF-1 function(s) were hypothesized to influence cell fate commitment?

Answer 13

CAF-1 alters chromatin structure and influences TF activity
(A)

Question 14

Which of the following is true of TFs in relation to hematopoiesis

Answer 14

TFs tightly control hematopoiesis AND TFs can change the fate of a cell
A and C (E)

Question 15

What does the loss of Chaf1nb, a subunit of CAF-1, mean for the lineage of primary cells of the Homozygous mice strain?

Answer 15

Loss of Chaf1nb results in unregulated cell differentiation and thus a mixed lineage state in primary cells
(B)

Question 16

Which of the following is true with respect to the significance of CAF-1

Question 17

When sustaining the current cell fate, what is the essential function?

Question 18

How did Franklin et al. determine a cell was differentiating from an iGMP to a Neutrophil?

Answer 18

More than one answer is correct (E)

Question 19

During a transcriptome analysis, with a CAF-1 OFF & HOXA9-OFF cell culture, which of the following is observed (true)?

Question 20

When a culture of cells expresses the wildtype CAF-1 function, what do we expect?