Presentations 3 and 4 Flashcards
In the manuscript, The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., what polymerase subunit is involved in facilitating H3-H4 parental histone tetramers to the leading strand of newly synthesized chromatin:
Pol epsilon (a)
In this article, what results were observed in parental histone transfer in mutant mcm2 cells with defective histone binding domains?
H3K4me3 showed a large reduction in parental histone transfer to the lagging strand
(C)
Which of the following is not a characteristic of ctf4?
A mutation in which of the following proteins resulted in the greatest reduction of H3K4me parental histone transfer to the lagging strand?
Ctcf4
(B)
In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers investigated H3-H4 histone transfer in ctf4Δ mutant cells, results of an eSPAN procedure indicated what?
Strong leading bias of H3K4me3 and strong lagging bias of H3K56ac
(C)
In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), four different mutant cell types were used, these included:
Mcm2-3A, ctf4Δ, ctf4-4E and pol1-4A cells
(D)
In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers utilized the RITE system. What does ‘RITE’ stand for?
Recombination-Induced Tag Exchange
(B)
In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers investigated H3-H4 histone transfer to the leading and lagging strands. What was the novel technique used in this study?
In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., which factors were found to facilitate the transfer of H3-H4 tetramers to the leading and lagging strands during chromatin replication:
Based on the study conducted in The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., it was proposed that future studies should look into:
Two of the above are correct
(E)
Histones and the post-translational modifications (PTMs) on them are inherited via:
The process of preparing chromatin (experimental rundown) to study the parental H3-H4 histone transfer involved:
______ histone (H3-H4)2 tetramers transfer to the _____ strand via Polε’s while transferring to the lagging strand via ______.
Recombination-induced tag exchange (RITE) system uses antibody tags to differentially tag parental and newly synthesized histones. This technique was used in mcm2-3A cells. Which tag represents which histone H3 modification in these mutated cells, and what is the impact of the strand bias?
It was noted through eSPAN peaks that ctf4Δ, pol1-4A, and mcm2-3A mutations all revealed similar strand bias patterns. What did Gan et al. observe when investigating the impact that the ctf4Δ, pol1-4A, and mcm2-3A mutations had on strand bias?
