Presentations 3 and 4

Question 1

In the manuscript, The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., what polymerase subunit is involved in facilitating H3-H4 parental histone tetramers to the leading strand of newly synthesized chromatin:

Answer 1

Pol epsilon (a)

Question 2

In this article, what results were observed in parental histone transfer in mutant mcm2 cells with defective histone binding domains?

Answer 2

H3K4me3 showed a large reduction in parental histone transfer to the lagging strand
(C)

Question 3

Which of the following is not a characteristic of ctf4?

Question 4

A mutation in which of the following proteins resulted in the greatest reduction of H3K4me parental histone transfer to the lagging strand?

Answer 4

Ctcf4
(B)

Question 5

In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers investigated H3-H4 histone transfer in ctf4Δ mutant cells, results of an eSPAN procedure indicated what?

Answer 5

Strong leading bias of H3K4me3 and strong lagging bias of H3K56ac
(C)

Question 6

In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), four different mutant cell types were used, these included:

Answer 6

Mcm2-3A, ctf4Δ, ctf4-4E and pol1-4A cells
(D)

Question 7

In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers utilized the RITE system. What does ‘RITE’ stand for?

Answer 7

Recombination-Induced Tag Exchange
(B)

Question 8

In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al. (2018), researchers investigated H3-H4 histone transfer to the leading and lagging strands. What was the novel technique used in this study?

Question 9

In the manuscript, The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., which factors were found to facilitate the transfer of H3-H4 tetramers to the leading and lagging strands during chromatin replication:

Question 10

Based on the study conducted in The Mcm2-Ctf4-Pola Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands by Gan et al., it was proposed that future studies should look into:

Answer 10

Two of the above are correct
(E)

Question 11

Histones and the post-translational modifications (PTMs) on them are inherited via:

Question 12

The process of preparing chromatin (experimental rundown) to study the parental H3-H4 histone transfer involved:

Question 13

______ histone (H3-H4)2 tetramers transfer to the _____ strand via Polε’s while transferring to the lagging strand via ______.

Question 14

Recombination-induced tag exchange (RITE) system uses antibody tags to differentially tag parental and newly synthesized histones. This technique was used in mcm2-3A cells. Which tag represents which histone H3 modification in these mutated cells, and what is the impact of the strand bias?

Question 15

It was noted through eSPAN peaks that ctf4Δ, pol1-4A, and mcm2-3A mutations all revealed similar strand bias patterns. What did Gan et al. observe when investigating the impact that the ctf4Δ, pol1-4A, and mcm2-3A mutations had on strand bias?

Question 16

Select the correct answer regarding Ctf4’s role in the replication fork.

Question 17

What two properties of recycled H3.3 and H3.1 parental histones are important to understand for their transmission and distribution?

Question 18

H3.3 colocalizes with ____-replicating chromatin and H3.1 with ___-replicating chromatin.

Answer 18

1) Early Replicating
2) Late Replicating
(B)

Question 19

Which histone chaperone is depleted to induce replication stress to visualise the impaired recycling of H3 variants?

Question 20

Hydroxyurea Treatment and Depletion of ASF1 both have similar implications when in comes to H3 variant histone recycling. These results are:

Answer 20

All of the above (e)

Question 21

Which of the following was already known before this research took place

Question 22

Which is false regarding the occupancy profiles of H3 variants during replication

Question 23

Which statement is true regarding H3.1 conglomerates throughout S-phase

Question 24

Which factor plays a key role in spatial distribution of H3 variants

Question 25

Replication stress was induced to investigate controlled histone recycling via:

Answer 25

Two of the above are correct (e)

Question 26

Which of these is true, with respect to global distribution of H3 variants during replication:

Question 27

H3.3 and H3.1 Genome-wide occupancy relative to replication timing revealed that:

Question 28

Replication stress via hydroxyurea (HU) treatment: