Presentations 1 and 2

Question 1

How does Taz1 regulate global replication timing?

Answer 1

Prevents the loading of Sld3 (e)

Question 2

What is the temperature sensitive allele that allows researchers to arrest cells at the G2/M boundary at high temperatures but also allows the synchronously release of cells at low temperatures?

Answer 2

Cell division cycle 25 (Cdc25) (a)

Question 3

Base substitutions to the telomeric repeats on the Replication Timing Control sequence will?

Answer 3

Induce early replication (d)

Question 4

4. Taz1 does not localize on…

Question 5

What is FALSE regarding Replication Timing Control?

Question 6

In the manuscript Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast by Tazumi, et al. Telomere binding protein Taz1 was found to regulate DNA replication timing control through which mechanism?

Question 7

In the manuscript Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast by Tazumi, et al. loss of the essential Replication Timing Control sequence has what effect on DNA replication at a Taz1 dependent origin?

Question 8

In the manuscript Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast by Tazumi, et al. which element was identified as a regulator of the global Replication Timing Control program in fission yeast S. pombe?

Question 9

Which factor was controlled to arrest the cells at G2/M boundary?

Question 10

How did Tazumi et al. discover that AT2088 have replication timing control (RTC) activity?

Question 11

What happens when the telomeric repeats of the essential sequence are mutated?

Question 12

How is late origin replication maintained?

Question 13

The control of DNA replication timing by chromatin organization is a known phenomenon, what other concept does the Tazumi et al., paper demonstrate to play a role in replication timing control?

Question 14

Which is the best statement to describe the relationship between Sld3 and Taz1?

Question 15

Which of the following have been shown to occur in taz1Δ cells (lacking Taz1 proteins) regarding the timing of replication?

Question 16

If there was a mutation in Taz1 resulting in a loss of function, which if the following would be expected to occur as a result?

Question 17

Which of the following is NOT a component of the replisome?

Question 18

What experimental technique was used to determine that Tof1 and Top1 have overlapping roles?

Question 19

Which of the following best describes the sTOP model of replication fork pausing proposed by Shyian et al.?

Question 20

Which of the following is a take-home message from the paper by Shyian et al.?

Question 21

What contributes to tumorigenesis and aging?

Question 22

Which of the following is true regarding Tof1-Csm3 and Rrm3?

Question 23

What suggested Tof1 and Top1 have overlapping roles?

Question 24

What does the Tof1-Csm3 complex recruit to the replisome?

Question 25

Dysfunctional replication fork pausing is thought to promote:

Question 26

It was found that Tof1 promotes:

Question 27

Which of the following are most correct regarding fork pausing complexes engaging topoisomerases at the replisome

Question 28

The sTOP mechanism proposes:

Question 29

The fork protection complex consists of :

Question 30

Which molecule functions independent of Tof1-Csm3 complex

Question 31

What is NOT a function of topoisomerase 1

Question 32

What is the sTOP mechanism