Preload Quiz 8 Flashcards
two main bacteriophage replication cycles
lyric and lysogenic
lysogenic
integrate into host genome as a propane
temporarily or permanently and replicate as bacteria cell replicates
doesn’t kill cell
lytic
bacteriophage injects its genome into host cell and induces replication of many copies of phage particle
phage particles released from host and cell lysis
prophage
a bacteriophage whose genome is injected into host DNA
how do bacteriophages infect their targets
through interactions with molecules on bacterial cell surface
what is bacteriophage T4R host
E. coli B
what is bacteriophage x174 host
e. coli C
what does a plaque assay fetermine
efficiency and nature of phage replication in a particular host
what is a plaque assay
virus particles and host cells are mixed together with soft agar and poured into agar plate
plaque
clear area produced by a virus particle that causes a productive infection
lawn
confluent growth
plaque forming units
phage numbers obtained from a plaque assay