PRELIMS 15% Flashcards
If there is a presence of ingested red blood cells, the E. histolytica is an?
invasive type
Infective stage of Amoebiasis
CYST
- Abdominal wall through rupture or open drainage of hepatic lesion
- Perianal or colostomy area
AMOEBIASIS ______
AMOEBIASIS CUTIS
AMOEBIC ___________
* Involvement of recto-sigmoid region causes frank dysentery.
AMOEBIC DYSENTERY
AMOEBIASIS _______
* Edematous elevation of skin
* Irregular margin of spreading ulcer
CUTIS
TROPHOZOITE of//?
- Cytoplasm is “dirty-looking” due to plenty of vacuoles that
contain ingested food particles as well as bacteria.
entamoeba coli
Motility of entamoeba coli TROPHOZOITE
SLUGGISH AND NONDIRECTIONAL WHEN ALIVE
DIAGNOSTIC CHARACTERISTIC OF ENTABOEBA COLI CYST
MULTIPLE PROMINENT NUCLEI
'’SMALL RACE”
ENTAMOEBA HARTMANI
MOTILITY OF E. HARTMANI IN TROPHOZOITE FORM
SLUGGISH AND SLIGHTLY DIRECTIONAL
DIAGNOSTIC FEATURE OF E. HARTMANI CYST
RICE-GRAINED CHROMOTOIDAL BODIES
ENDOLIMAX NANA MOTILITY IN TROPHOZOITE FORM
“sluglike” motility
DIAGNOSTIC FEATURE OF ENDOLIMAX NANA CYST
CROSS-EYED APPEARANCE OF THE 22 ADJACENT NUCLEI
COMMA-SHAPED CHROMOTOIDAL BODIES
GROUND GLASS CYTOPLASM
Its trophozoite form has a Cytoplasm with a large pinkish glycogen mass that is about
½ or 1/3rd of the size of the organism.
iodamoeba butschili
LARGE GLYCOGEN MASS ALMOST OCCUPYING THE SIZE OF THE ORGANISM is the diagnostic characteristic of?
iodamoeba butschili in CYST form
_________ causes primary amoebic meningoencephalitis (PAM).
Naegleria fowleri
- Also known as the brain-eating amoeba.
- This amoeba is commonly found in warm freshwater and soil.
NAEGLERIA FOWLERI
The recommended treatment for Naegleria infection is a
combination of ?
AMPHOTERICIN B and MILTEFOSINE
They are also obtained from Jacuzzis, air-conditioning and ventilating units, dialysis machines, and even contact lens.
ACANTHAMOEBA SPECIES
Cyst: is double walled with polyhedral or stellate endocyst
and wrinkled ectocyst.
ACANTHAMOEBA ___________
ACANTHAMOEBA POLYPHAGA
Cyst: biconcave in shape with a folded ectocyst and stellate
endocyst.
ACANTHAMOEBA ___________
A. ASTRONYXIS
A. polyphaga and A. hatchetti were found in cases of ??
EYE INFECTIONS ONLY
How many stool samples should be collected when following the
typical O&P collection protocol?
3
the ratio of fixative to stool specimens
3:1
All-purpose fixative for the recovery of protozoa and
helminths
FORMALIN
Comprised of a plastic powder that act as an adhesive for
stool specimen when preparing slide for staining.
POLYVINYL ALCOHOL
- Can be sued for performing conc techniques and permanent
stained smear. - Only requires single vial and it is mercury free
SODIUM ACETATE FORMALIN
- Free of formalin and mercury
- Can be sued for conc techniques and
permanent stained smears.
ALTERNATIVE SINGLE-VIAL SYSTEM
Diagnostic stages of parasites
detected microscopically are measured in units known as
??
MICRONS
Also known as direct well mount.
DIRECT WET PREPARATION
- May be made to enhance the detail of the protozoan cysts.
- Lugol’s or D’Antoni’s formula (drop of iodine)
- It destroy the TROPHOZOITES stages of protozoan.
DIRECT WET IODINE PREPARATION
clearing solution in KATO THICK SMEAR
GLYCERINE
used to give color to the cellophane in
order to give a pale green bg to the eggs and to minimize the
brightness of the microscopic field
MALACHITE GREEN
- Useful in detecting eggs with thick shells such as Ascaris
and Trichuris. - Not eggs with thin shells (hookworm)
KATO-THICK SMEAR
Reagents:
o 40% HCl
o Ether
ACID ETHER CONC TECHNIQUE
- Most widely used sedimentation techniique.
- Based on specific gravity
FROMALIN-ETHY ACETATE SEDIMENTATION PROCEDURE
The direct wet preparation can be eliminated from the O&P
examination if the specimen is received in fixative.
TRU OR FOLZ
TRUE
Useul for the recovery of both helminth eggs and protozoan
cysts.
* Makes use of 10% formalin which is all purpose fixative
FORMALIN ETHER CONCENTRATION
Zinc sulfate with a specific gravity of ???
1.18 to 1.20
- Is considered as the BEST for the recovery of coccidian oocysts,
mainly Cryptosporidium, Cyclospora and Isospora.
SHEATHER’S SIGAR FLOATION
Boiled sugar solution preserved with phenol is used in this
method.
SHEATHER’S SIGAR FLOATION
- Time-consuming
- Excellent morphology of the intestinal protozoa.
IRON HEMATOXYLIN
- Most widely used permanent stain
- It uses reagents with a relatively long shelf life and the procedure is easy
to perform
WHEATLY TRICHOLME
- Use of a saturated table salt solution.
BRINE FLOTATION
- Positive stools are mixed with moistened soil or granulated
charcoal.
BAERMANN TECHNIQUE
COPRO CULTURE
Consistency of the stool is the main determinant for the
sensitivity of this technique.
Used for assessing the intensity of infection in
Schistosomiasis and common soil transmitted
helminthiases
KATO KATZA METHOD
STOOL DILUENT IN STOLL EGG COUNT IS
SODIUM HYDROXIDE
- Is the specimen of choice for the detection of Enteriobius
vermicularis (pinworm) eggs. - Otherwise known as the Graham technique
CELLOPHANE TAPE PREPARATION
- A gelatin capsule that contains a coiled length of yarn should be
swallowed by the patient.
DUODENAL MATERIAL
Blood parasites that may be recovered in the blood:
▪ L___________
▪ Trypanosoma spp.
▪ P________
▪ Babeisa spp.
▪ M________
▪ Leishmania donovani
▪ Trypanosoma spp.
▪ Plasmodium
▪ Babeisa spp.
▪ Microfilaria
most useful in species identification of malarial
parasites.
THIN SMEARS
used in the demonstration of microfilariae and
rapid diagnosis of malarial infection.
THICK SMEARS
o Considered the preferred stain because it allows for the detection of parasite detail necessary for species identification
o May be prepared from powder or may be commercially purchased a conc stock solution.
GIEMSA STAIN
Makes use of a capillary tube which is precoated with acridine
orange and potassium oxalate.
QUANTITATIVE BUFFY COAT
Designed to concentrate blood specimens suspected of
containing low number of microfilariae
KNOTT’S CONCENTRATION
- Also useful when the density of microfilariae is low.
- Make use of syringe attached to a Swinney filter holder
MEMBRANE FILTRATION
is an examples of culture
medium designed for the recovery of Leishmania spp. and
Trypanosoma cruzi
NOVY-MACNEAL-NICOLLE
Thick blood smears for malaria are recommended for species
identification?
T OR F
FALSE
Produces antibodies that can cause
precipitation reaction on the eggs of
the parasites
CIRCUMOVAL PRECIPITIM TEST
RETRACTED CYTOPLASM IS A DIAGNOSTIC FEATURE OF
GIARDIA DUODENALIS CYST
“OLD MAN WITH EYE-GLASSES APPEARANCE” CAN BE SEEN IN
GIARDIA DUODENALIS TROPHOZOITE
giardiasis causes traveler’s diarrhea and
called as ?
“Leningrad’s curse”
Cytoplasm is filled with numerous siderophile
granules (diagnostic) is present in
TRICHOMONAS VAGINALIS TROPHOZOITE
It exists both in cyst and trophozoite form, however it is often
found solely as a trophozoite.
DIENTAMOEBA FRAGILIS
Round, ovoid, or shaped like a torpedo (referred as torpedo
form), singly nucleated, non-flagellate, with prominent
kinetoplast and axoneme.
AMASTIGOTE
Elongated with pointed ends, singly nucleated, with
prominent kinetoplast and axoneme with anterior flagellum.
PROMASTIGOTE
An intracellular parasite that cause the disease
leishmaniasis, Kala-azar, death fever and Dum-dum fever
LEISHMANIA DONOVANI
It is intracellular and non-motile form because
it lacks external flagellum.
AMASTIGOTES
Chagas disease can be treated with benznidazole and
_____________
nifurtimox