PRELAB LIPID EXTRACTION FROM PC Flashcards
Weigh _.__g of potato chip sample.
5.00g
Add 20mL of _________ _____ into the mortar and continue crushing.
petroleum ether
Formula for theoretical mass of fat extracted:
THEROTICAL FAT EXTRACTED(G) = MASS OF SAMPLE / MASS OF SERVING SIZE X TOTAL FAT
Formula for the percent difference of the weight of the dried residue with that of the amount of total fat per serving indicated in the nutritional facts
%DIFFERENCE = (THEORITICAL FAT (G) - MASS OF RESIDUE (G) / THEORITCAL FAT (G) X 100
Reagents Used
Petroleum ether
Objectives
- Extract lipids from a chosen food sample
- Identify the appropriate solvents for the extraction of lipids
- Determine the difference of total fat content extracted and total fat indicated in the nutritional facts of the food sample
Title of the experiment
LIPID EXTRACTION FROM POTATO CHIPS
Class of biomolecule that are diverse in terms of structures
LIPIDS
What makes these structurally diverse molecules fall in one group is their…
Insolubility in polar solvents
EX: water and alcohols
Composed mainly of fatty acids and triacylglycerides (TAGs)
DIETARY LIPIDS
Fatty acids are used as starting materials for
MEMBRANE LIPIDS
MESSENGER LIPIDS
Some fatty acids are used as starting materials for
membrane lipids and messenger lipids particularly the __________
Eicosanoids
Also serve as a membrane lipid and a precursor of steroid hormone
CHOLESTEROL
lipids can also serve as
SCENTS
FLAVORINGS
DYES IN FOOD
PHARMACEUTICALS
Studying the chemical properties and biochemical significance of lipids begin with the extraction from biological or biological-based materials such as…
PLANT TISSUE
FOOD