PRELAB Analysis of Urine

Question 1

Name of Experiment

Answer 1

Analysis of Urine

Question 2

Normal urine is mostly water with about ____% dissolved substances

Answer 2

Five

Question 3

Benedict’s Test for Sugars

Answer 3

Checks for colored precipitates

Concentration of sugars in urine

Question 3

Biuret Test for Proteins

Answer 3

Purple colored-product

Confirms the presence of protein

Question 3

label each tube as
U for _____
S for _________
C for _______

Answer 3

Urine

Standard

Control

Question 4

Test for Ketone Bodies

Answer 4

Check for the formation of a PINK to PURPLE ring

Question 5

GRADE RESULTS FOR

Pinkish Ring:

Red ring:

Deep Purple Ring:

Answer 5

+

++

+++

Question 6

Test for Bicarbonate

Answer 6

Formation of gas bubbles

Proves the presence of carbonate and/or bicarbonate.

Question 7

Test for Chloride

Answer 7

A white precipitate

Confirms the presence of chloride

Question 8

Only the edges of the paper will turn
blue and will happen slowly

Answer 8

If a small amount of ammonia

Question 9

Will quickly turn all of the paper blue.

Answer 9

A larger amount of ammonia

Question 10

Concentration for Proteins :

No difference when looking down through tube -

Faint violet – looking down but not looking
horizontally -

Faint violet when looking horizontally -

Clearly violet, even when not compared with
blank -

Dark purple -

Answer 10

0-20

20-100

100-300

300-500

> 500

Question 11

Concentration for sugar :

blue to blue-green (with no ppt.) -

green with small quantity of yellow ppt. -

olive green -

yellow to orange -

brick red -

Answer 11

0-40

40-50

50-100

100-200

> 200