Pre-lab assignment #1

Question 1

Define PPE?

Answer 1

PPE is Personal Protective Equipment

Question 2

What is the function of PPE

Answer 2

To keep hazardous materials off of your skin and protect you from serious or fatal chemicals. To avoid skin exposure.

Question 3

What is the proper dress code in a lab (name 3-4 specifics)?

Answer 3

a. Long pants
b. Closed toed shoes
c. Remove jewelry
d. Tie back long hair

Question 4

Give 3 examples of PPE commonly used in a lab?

Answer 4

a. Chemical Apron
b. Safety glasses/goggles
c. Gloves

Question 5

In the past couple of years, another PPE, which is not mentioned on this video because most commonly used by medical personnel, has been recommended by public health officials to prevent the spread of respiratory infections, such as COVID-19. What PPE is this?

Answer 5

Masks were the PPE used to protect against COVID-19

Question 6

How can you accidently spread biological material and cause contamination?

Answer 6

By touching items like light switches, phones and door knobs while still wearing your gloves

Question 7

What are 4 types of safety equipment that should be in EVERY lab?

Answer 7

a. Safety Shower
b. Eye wash station
c. Fire extinguisher
d. Evacuation Routes

Question 7

What steps should be taken if a person is exposed to a flame or chemical?

Answer 7

Yell for help and immediately move to the nearest safety shower. Remove the saturated clothing and drench the affected skin under the shower, if it is your clothes or skin that are exposed to flames, drench the whole body and have someone call 911.

Question 8

What steps should you take when you leave the lab?

Answer 8

Always remove your PPE and wash your hands before leaving

Question 9

What steps should be taken if a person gets a chemical in their eye?

Answer 9

Hold your eyes open and thoroughly rinse them for at least 10 minutes under the eyewash station

Question 10

List the five environmental conditions necessary to grow bacterial cells in lab

Answer 10

a. Temperature
b. Moisture
c. pH
d. Oxygen
e. Nutrients

Question 10

What are the four types of fire? Describe each?

Answer 10

a. Class A fires: they are fires that consist of ordinary combustibles like wood, cloth, paper. They can be extinguished by water or extinguishers.

b. Class B fires: Fires that include organic solvents and flammable liquids. Must be extinguished with an extinguisher not water.

c. Class C fires: Fires that involve electrical equipment. Must be extinguished with an extinguisher not water.

d. Class D fires: Fires that involve combustible metals which aren’t very common in the lab

Question 11

What are four behaviors that should NEVER occur in a lab?

Answer 11

a. Never Eat
b. Never Drink
c. Never chew gum
d. Never apply makeup

Question 12

Which type(s) of fires can be extinguished by a typical fire extinguisher found in a lab?

Answer 12

Class A, B, and C fires

Question 13

When and how should a fire blanket be used?

Answer 13

Can extinguish small fires on work benches or the floor, can also be used to help someone whose clothing is on fire. DO NOT wrap them while they are standing up, help them to the floor and wrap them in the blanket and help them roll around until the fire is out

Question 14

Provide two benefits of autoclaving your media?

Answer 14

a. Safety: it ensures it is safe to consume without contaminants and Replication: it allows the research to be replicated to make sure the results are accurate

Question 15

How should broken glass be handled?

Answer 15

Sweep the glass, do not pick it up by hand and dispose of it in a broken glass container

Question 16

How should a bench be cleaned before and after use?

Answer 16

You should wipe it down with isopropyl alcohol or ethanol before working and keep it organized and clean as you go. When finished you should wash and dry any and all of the dishware used, return reagents to their storage areas, clean the work bench with isopropyl alcohol or ethanol.

Question 17

What is solid media?

Answer 17

a. Solid media are used to grow microorganisms in their full physical form and prepare bacterial pure cultures, or isolate bacteria to study colony characteristics.

Question 17

What is an autoclave and how does it work?

Answer 17

a. An autoclave a method of sterilizing heat-stable culture media is known as steam sterilization. This method kills microorganisms by subjecting it to a temperature of 121°C at 15 pounds of pressure for 15 minutes. The steam under pressure is a very effective way to sterilize the media.

Question 17

Once sterile agar is poured into plates and has solidified, why do we store plates upside down? Provide two reasons

Answer 17

a. To Prevent contaminants in the air within the plate from settling on the agar surface

b. So condensation doesn’t form on the underside of the lid and fall onto the agar and compromise the culture