PRE-ANALYTICAL FACTORS AND GROSS DESCRIPTION Flashcards
3-5mm thick tissue is fixed for how long
6-48 hrs
tissues are sectioned prior to fixation (size)
5mm thick TSE and large TSE
1st and most important step in histopath outside the tissue processing procedures
Specimen accessioning
Indicating cofes
Surgical
autopsy
Cytology
Format of Accession Number
Code-year-ID- id num of specimen
Specimens only requiring transfer from container to tissue cassette
Endometrium
Breast core biopsies
Colonic series
Specimens requiring transfer but with standard sampling, counting, weighing or slicing
Small lipoma
Small skin biopsy
Cervical LLETZ
Simple dissection required with sampling needing a low level of diagnostic assessment and/or preparation
Prepuce
Gall bladder
Hemorrhoids
Appendix
Dissection and sampling required needing a moderate level of assessment
(PigLaSS)
Pigmented skin lesions
Large intestine
Skin w markers
Salivary gland tumour
Specimens requiring complex dissection and sampling methods
Thyroid
Breast cancer
Testis
Uterus
Excess finger/toe will be removed and will be submitted to the histopath lab for description only
Accessory digits
Bony bump that forms at the joints of the base of the big toe
Bunions (hallux valgus) & hammer toes
Medical devices that are excluded from mandatory submission
Catheters
Gastrostomy tubes
Stents
Sutures
do not meet with the criteria of the hospital or laboratory for examination
Placentas
there is an alternative policy for documentation for these types of samples during surgical removal
Orthopedic hardware and other radiopaque medical devices
removed only for purposes of gaining surgical access but is not suggestive of any form of malignancy
Rib segments or other tissues
harvested from a coronary artery bypass
Saphenous vein
used to identify and orient the specimen’s components, distinguish samples, for embedding instructions
Inks
Indicates laterality
Nicks
Represented by LL long lateral or SS short superior
Sutures
to accurately and faithfully transmit information to allow accurate and reliable microscopic assessment of this margin
Inking
set as an instruction for the embedding process
Inking
instruct the embedder to embed the tissues a certain way
Ink dots
Used for ink dots
India ink
Used to remove ink
Acetic acid
Dimensions are rounded to neares
Length width depth
- 1cm
Measurement of standard cassette
3 x 2.5 x 0.4 cm
Thickness of TSE allowed in standard cassette
Not more than 0.3 cm
Edges of tissues should be
Squared
Small specimens are cut
Serially about 2mm thick
Used got wrapping small sample
Filter paper
Large specimens are cut
Interval of 1cm thickness (breadloafing)
en bloc excision of all cancer bearing tissues in the pelvis including the bladder, the prostate
Cystoprostatectomy
embedded in the cassette for labeling
Paper tags using lead pencil
If printed what is used for labeling
Dot matrix
Brain is tied at the
Circle of willis
What is circle of willis
CIRCULATOIN where BLOOD FLOWS or the pathway of the blood to supply oxygen to the brain as well as the surrounding structures
Brain is imemrsed in
10% neutral buffered formalin for 2-3 weeks
Base of colon cancers are called
Polyps
area where cautery arteries are located
Polyps
bisected and
places in one cassette
Small polyps
sides are trimmed away from the stalk, and stalk is placed in a separate cassette
Large polyps
Orienttion of dermatologic specimen
Vertical
Dermatologic specimen “-“ are submitted whole
Punch biopsies
Tissues greater than - are dissected
4mm
serially cut along the short
axis at 2 to 3 mm interval.
Skin ellipses
Wash in running water then immerse in TSE softeners
Must be decalcified first
Hard tissues
cut open longitudinally and fixed with cottons inside
Hollow structures
Most important component of tumor resections
Lymph nodes
essential for prognosis and planning therapeutic options
Lymph nodes
received fresh and not immersed in formalin
Lymph nodes
first lymph node to be involved during metastasis. Entirely submitted.
Sentinel lymph nodes