Decalcification Flashcards
Routine or most common decalcifying agent
● Fastest decalcifying agent in the market now
Nitric acid
Remedy when nitric acid formes yellow coloration
Add urea or sodium thiosulfate
Acts as tissue softener
Perenyi’s fluid
Nitric acid variation
Fastest agent
Phloroglucinol-nitric acid
Provides good nuclear staining at 1%
● Slower and causes more distortion
Hydrochloric acid
Hydrochloric acid variation for For teeth and small bones
Von ebner’s fluid
Good for ROUTINE DECALCIFICATION of post-mortem research tissues, small pieces of bone and teeth, ISH staining
Formic acid
Best for small bone spicules
● Good nuclear staining
● Slow, weak
Trichloroacetic acid
Best for minute bone spicules
Chromic acid (flemming’s fluid)