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HISTOPATH LEC MIDTERMS > Microtomy > Flashcards

Microtomy Flashcards

1
Q

Rocking microtome was invented by

A

Paldwell trefall

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2
Q

Thickness of cambridge

A

10-12 um

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3
Q

Tissues are cut in slightly curved planes thus not recommended for serial sections

A

Rocking micro

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4
Q

Most common and its thickness

A

Rotary; 3-5 um

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5
Q

Used for routine and research laboratories

A

Rotary

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6
Q

Rotary invented by

A

Minot 1885-86

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7
Q

Knife and block holder are brought together by upward and vertical motions, cutting sections in a perfectly flat plane;

A

Rotary

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8
Q

Sliding microtome developed by

A

Addams 1789

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9
Q

√ electrically
driven and ideal for RESIN-EMBEDDED
decalcified bone.

A

Base sledge microtome

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10
Q

knife is moving; the MOST DANGEROUS type of microtome because of the exposed, movable blade.

A

STANDARD SLIDING MICROTOME

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11
Q

Recommended for cutting “extremely” hard and rough tissue blocks

A

SLIDING

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12
Q

Recommended for cutting “extremely” hard and rough tissue blocks

A

SLIDING

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13
Q

Both Microtome, the knife can be set OBLIQULEY for celloidin or straight large paraffin.

A

SIIDING

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14
Q

Freezing microtome invested by

A

Quickett 1848

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15
Q

Freezing agent of freezing microtome

A

Co2

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16
Q

Used to cut undehydrated tissues in a frozen state.

A

Freezing micro

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17
Q

Ideal for fresh tissues that did NOT UNDERGO FIXATION down to INFILTRATION.

A

FREEZING MICRO

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18
Q

Used for RAPID preparation of URGENT tissue biopsies
for intraoperative diagnosis (STAT).

A

CRYOSTAT

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19
Q

Chamber of cryosat temp

A

-5 to -30C

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20
Q

Capable of freezing fresh tissue within 2-3 minutes.

A

Thermostat

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21
Q

Cutting section of thermostat

A

4um

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22
Q

Fluorescent antibody staining technique or histochemical enzyme studies.

A

Cryostat

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23
Q

For electron microscopy, tissues fixed with osmic
acid.

A

Ultrathin micro

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24
Q

Media of uultrathin micro and thickness

A

Plastc; 0.5 um

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25
Q

Uses diamond knife

A

Ultrathin

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26
Q

Types of tissue sections

A

Paraffn
Celloidin
Frozen sections

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27
Q

Sections are removed in RIBBONS OF TEN to allow easy location of serial sections

A

Paraffin

28
Q

Thickness of paraffin

A

4-6um

29
Q

Thickness of celloidin

A

10-15 um

30
Q

To avoid dehydration and shrinkage, sections are usually cut by the WET method,

A

CELLOIDIN

31
Q

In celloidin, sections and the block being kept moist with — during cutting.

Sections do not come off in -

A

70% alcohol

Ribbons

32
Q

Microtome knives

A

Plane-concave
Biconcave
Plane-wedge

33
Q

Widely use blade for 10 tissue blocks

A

Disposable blades

34
Q

Disposable blade is coated w

A

Polytetrafluoroethylene

35
Q

For ultrathin microtomes.

A

Glass knive/ralph knive

36
Q

For RESIN blocks on ultrathin
microtomes;
● Brittle and expensive

A

Diamond knives

37
Q

Blades For partially calcified materials, paraffin, and frozen sections.

A

Safety razor blades

38
Q

Blades

Unsatisfactory for sections less
than 10 micro.

A

Safety razor blade

39
Q

Bevel angle

A

27-32

40
Q

Cutting angle

A

14

41
Q

Angle between the UPPER facet of the knife and the SURFACE of the tissue block.

A

Cutting angle

42
Q

Angle between the LOWER facet of the knife and the SURFACE of the tissue block;

A

CLEARING ANGLE

43
Q

Clearing angle should be inclined at

A

5-10

44
Q

2 stages of sharpening

A

Honing
Stropping

45
Q

Removing of GROSS nicks

A

Honing

46
Q

HEEL TO TOE

A

HONNING

47
Q

TOE TO HEEL

A

STROPPING

48
Q

Final polishing of the knife edge

A

STROPPPING

49
Q

Removal of burr/irregularities

A

STROPPING

50
Q

Knife sharpeners for finall polishing

A

Diamantine

51
Q

— is used in stropping

A

Paddle strop

52
Q

Never used with Pap Staining Method as it will retain OG stain;

A

Mayer’s egg albumin

53
Q

Outdated blood stored in blood banks, dispensed into sterile tubes of 0.5 ml each.

A

Plasma

54
Q

Better adhesion than albumin

A

1% Gelatin

55
Q

Does NT RETAIN the stain.

A

1% METHYL CELLULOSE

56
Q

ADHESIVE

No background staining

A

Poly-L Lysine

57
Q

Slight dye retention, not affected by mild alkaline solutions 3 months.

STRONG ADHESIVE

A

SODIUM SLICATAE

58
Q

Diluted 1:10 with acetone;

A

RESIN

59
Q

Selected sections for staining should fish-out in a — POSITION

A

VERTICAL

60
Q

Sections are floated out on a water bath set at

A

45-50°C.

61
Q

Temp of slide dryer

A

5-10°C above the melting point of paraffin

62
Q

In drying slides, hot plates are not recommended because

A

Overheating
Dust falling onto section

63
Q

Mounted sections are placed in — for - miutes

A

Paraffin oven; 5 min

64
Q

Process of trim and embedding

A

Trim tissue
Cool block w ice
Cut tissue -> make ribbons
Float tse in water bath
Pick up tissue on slide
Dry

65
Q

Ideal shape in trimming

A

Truncated pyramid

HISTOPATH LEC MIDTERMS (5 decks)

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