Practice Questions Exam 1

Question 1

What is the role or RecA in homologous recombination

Answer 1

it allows single strand DNA binding to double strand DNA

Question 2

What is a Holliday junction?

Answer 2

DNA intermediate containing 4 strands of DNA from 2 different helices

Question 3

Why is it important that there is a branched point consensus sequence in the pre-mRNA?

Answer 3

it provides a similar site that is unique to each intron for snRNP’s to bind for splicing

Question 4

During meiotic recombination, what allows crossover to occur?

Answer 4

if the strands of the double Holliday junction are cut in opposite directions

Question 5

Is TATA upstream or downstream of BRE?

Answer 5

Downstream

Question 6

Which RNA polymerase transcribes mRNA?

Answer 6

RNA polymerase II

Question 7

name the structure the DNA only transposons form in order to move to a different gene

Answer 7

transposome

Question 8

True or False: The aborted RNA causes variation in the desired protein product

Answer 8

False

Question 9

name a type of DNA damage that DNA helicase would be recruited to assist with the repair

Answer 9

Pyrimidine dimer because nucleotide excision repair would need to occur and helicase removes the dimer after nuclease cuts it out of the sequence

Question 10

What is the role of glycosylase in DNA repair and which repair pathway is it involved in?

Answer 10

it flips out bases one by one while looking for errors; base excision repair

Question 11

What are the characteristics of short telomere syndrome?

Answer 11

Gray hair at a young age

recurrent infections

jaundice

Question 12

Xeroderma Pigmentosum is an example of what type of defective mechanism?

Answer 12

Defective nucleotide excision repair

Question 13

You see a young girl playing outside at the park and she catches your eye. You notice she seem small for her age with abnormally small head and rosy cheeks. what condition might she have?

Answer 13

Bloom Syndrome

RecQ helicases are afflicted so the DNA helicase needed for recombination is afflicted as well

Cancer at several sites, stunted growth, genome instability Beak nose, butterfly rash, disproportionately small head
Autosomal recessive

Question 14

name a type of DNA damage that DNA helicase would be recruited to assist with the repair

Answer 14

Pyrimidine dimer because nucleotide excision repair would need to occur and helicase removes the dimer after nuclease cuts it out of the sequence

Question 15

What type of transposon is characterized by a structure having long terminal repeats at each end?

Answer 15

Retroviral-like retrotransposons

Question 16

Describe heterochromatin in terms of structure and genetic activity

Answer 16

tightly packed and genetically inactive

Question 17

microRNA’s have tremendous potential for disease intervention in nearly all cases where stopping gene activity would be valuable. name a potential cause for concern for the use of miRNA’s for therapeutic intervention

Answer 17

precise delivery to tissue of significance

off target interactions