Practicals - Paper 1 Flashcards
Investigate how enzyme activity changes with temperature
Amylase breaksdown starch to maltose
Test for starch using iodine
Vary temperature of amylase usign a water bath
Iodine solution in wells and add different temp amylase
Investigate Osmosis in living systems
potato cubes in salt solution
Practical - osmosis in living system
- Cut 2cm potato cubes
- Measure mass of potato cubes
- Measure out 15cm of salt solution - vary concentration
- Place potato cube in different salt solutions
- Wait 30 minutes
- Remove potato from solution
- Measure new mass
Investigate osmosis in non-living systems
Visking Tube
practical - osmosis in non-living system
- Vary concentration of sucrose solution in visking tube
- Place Visking tube in distilled water
- Measure mass of visking tube before and after
- Repeat each concentration three times
Investigate how light intensity changes rate of photosynthesis
- Change distance between light and pondweed
- Measure number of O2 bubbles produced
Investigate production of starch in a Leaf
- leaf in boiling water - stop chemical reactions
- put leaf in ethanol and heat in water bath - to remove chlorophyll
- Add Iodine solution
- Positive result = orange to blue/black
If leaf is in dark
Uses up starch to photosynthesis
No starch present
Investigating effects of Chlorophyll on photosynthesis
- Expose a variegated leaf to light
- Test leaf for starch
- Only the green parts turn blue/black
- only parts containing chlorophyll are able to photosynthesise
Investigate the effect of CO2 on photosynthesis
- Place plant in bell jar wiht lime soda
- lime soda absorbs CO2
- Leave plant in jar
- Test for starch
- no starch proves that CO2 is needed for photosynthesis
Hydrogen carbonate indicator
Shows concentration of CO2
Red solution
Hydrogen Carbonate indicator in hich CO2 conc
Turns yellow
Conditions needed for seed germination
Warmth - Optimum Tmeperature for enzymes
Oxygen - Aerobic Respiration
Water - Activate enxymes
Aerobic respiration
Exothermic Reaction - realeases heat
Releases CO2
Investigate the population size of an organim in two different areas using quadrats
- Divide the area you are sampling into a grid using a tape measure
- Use a random number generator to randomly generate coordinates using the random sampling method
- At each coordinate, place the quadrat down and count the number of organisms in each quadrat
- Repeat this process multiple times (20 or more) so that the sample size is representative of the whole area
How do you calculate population size of an area
(Average number of organism per quadrat/Size of quadrat) x size of whole area
