Practical Microbiology

Question 1

what is an asceptic technique

Answer 1

without microbes

prevention from contamination

Question 2

what are diluents needed for

Answer 2

needed to dilute or suspend microbes, hold them in suspension

Question 3

name 6 sterilising techniques

Answer 3

autoclaving, flaming, alcohol flaming, filtration, radiation, chemical

Question 4

describe autoclaving

Answer 4

pressurised steam steriliser, heats water under pressure
121 degrees, 15 min , 15 psi
used for glass wear and equipment only
targeting endosperm

Question 5

what is advantage of flaming

Answer 5

it cools fast

Question 6

HEPA

Answer 6

type of air filters, sterilise air in clean room or cabinets

Question 7

laminar flow cabinet

Answer 7

air is passes through a HEPA filter

Question 8

class 2 cabinet

Answer 8

working with potential pathogens

inflow and down flow

Question 9

name routs of infection

Answer 9

oral, dermal, inhalation

Question 10

what is fermenter used for

Answer 10

to grow larger volumes
contain electrons to measure O2 and pH
temperature control

Question 11

continuous culture

Answer 11

at log phase constantly,
all conditions remain the same
chemostat - continuous culture growth, limited by 1 of the medium components