practical laboratory immunology

Question 1

extractable nuclear antigens

Answer 1

Ro, La: SLE and sjogrens
RNP: SLE and MCTD
Scl70: systemic sclerosis
Sm: SLE
Jo-1 polymyositis and dermatomyositis
ANA: SLE

double stranded DNA SLE

Question 2

tryptase

Answer 2

acutely elevated following systemic mast cell degranualtion

timing of samples is critical (immediate, within 4 hrs, over 24hrs)

Question 3

total IgE

Answer 3

elevated in atopic disease, parasitis infection

Question 4

immunoglobulin measurements

Answer 4

IgG, IgA, IgM

indications- only 2 main ones
investigation of suspected myeloma or immunodeficiency

Question 5

myeloma

Answer 5

IgG
IgA
IgM

Question 6

coeliac

Answer 6

TTG
if +ve = coeliac disease (biopsy remains a gold sandard)

if -ve this can be either the patient does not have coeliac, the patient does have coeliac but is on a gluten free diet or the patient does have coeliac but is IgA deficient