Practical knowledge Flashcards
1
Q
What is ODC rate limiting for?
A
biosynthesis of polyamines
2
Q
What does the ribosomal frameshift have to do with OAZ1 maturation
A
OAZ1 has two ORF and without the frame shift there is a premature stop codon -> Oaz1 is unable to be expressed
3
Q
what is the tetracyclin chase experiment?
A
Aptamere at beginning of Construct froms secondary structure upon addition of Tetracyclin -> blocks translation and steady state protein level can be measured.
4
Q
What is cdc48
A
A ubiquitin specific segregase. Involved in DNA repair and autophagy
