Practical: Investigating The Antibacterial Properties Of Plants

Question 1

Describe some aseptic techniques

Answer 1

-sterilise inoculation loop by holding it in blue flame of Bunsen burner before and after handling bacteria
-flame neck of flask containing bacteria
-minimise time with lid off to prevent contamination
-disinfect work surfaces
-glassware sterilised in an autoclave
-work near Bunsen burner which will create convection currents of hot air to kill bacteria in air

Question 2

Why inoculate at 25 degrees Celsius in school lab but 37 degrees Celsius in commercial lab

Answer 2

At 25 degrees Celsius to prevent harmful pathogen growth, grown at 37 degrees Celsius as this is optimum temperature for enzyme action and is temp in human body

Question 3

What does accuracy mean

Answer 3

How close a result is to the true value

Question 4

What does precise mean

Answer 4

How close together the results are