Practical 4: Enzyme and substrate concentrations - rates of reactions

Question 1

Enzyme and substrate concentrations - rates of reactions

Answer 1

1. Dilute stock solution of trypsin with distilled water to produce solutions with
   concentrations of 0.2%, 0.4%, 0.6% and 0.8%.
2. Make a control by adding 2cm3
   of trypsin solution and 2cm3
   of distilled water.
   Use this to set the colorimeter absorbance to zero.
3. To another cuvette, add 2cm3
   of milk suspension and 2cm3
   of the stock trypsin
   solution. Mix, place in the colorimeter and measure absorbance at 15 second
   intervals for 5 minutes.
4. Rinse the cuvette with distilled water.
5. Repeat step 3 at all trypsin concentrations.

risks- broken glass, hot liquids

variables-
control = volume of solution
independent = concentration of solutions
dependent = rates of reaction

plot on a graph