Practical 12: Temperature and the rate of an enzyme catalysed reactions

Question 1

Temperature and the rate of an enzyme catalysed reactions

Answer 1

1. Grind a known mass of peas in distilled water and place in a boiling tube.
2. Add 5cm of hydrogen peroxide solution to the peas.
3. Fit the syringe into a delivery tube and the delivery tube into the boiling tube with
   a bung.
4. Place the boiling tube into a water bath at a known temperature.
5. Time for a set length of time e.g. 5 minutes. Measure the volume of gas
   produced at regular intervals e.g. 30 seconds.
6. Repeat the experiment at different temperatures.

risks- broken glass, hot liquid

variables-
control = volume of hydrogen peroxide solution
independent = temperature
dependent = rate of reaction

plot the data in a graph