Practical 1 Haematology Flashcards
1
Q
Average blood volume of adult animals
A
60-80 ml/kg bwt
2
Q
Density of blood
A
1050 g/dm3
3
Q
relative viscosity of blood
A
3.5
4
Q
Ph of blood
A
7.35 - 7.45
5
Q
Osmolarity of blood
A
300 mosmol/l
6
Q
osmolality of blood
A
300 mosmol/kg
7
Q
total osmotic pressure of blood
A
700 kPa
8
Q
oncotic of blood
A
3.5 kPa
9
Q
freezing point of blood
A
-0.56
10
Q
hemoglobin concentration of blood
A
120 - 180 g/l
11
Q
Density of blood plasma
A
1020 g/dm3
12
Q
Na+ conc of blood plasma
A
140mmol/l
13
Q
k+ conc of blood plasma
A
4mmol/l
14
Q
Mg2+ conc blood plasma
A
1.5mmol/l
15
Q
Ca+ conc mamanal blood plasma total
A
2.5mmol/l
16
Q
Ca+ conc mamanal blood plasma ionic
A
1.25mmol/l
17
Q
Ca+ conc poultry blood plasma total
A
5 mmol/l
18
Q
Ca+ conc poultry blood plasma ionic
A
1.25mmol/l
19
Q
cl- conc blood plasma
A
110 mmol/l
20
Q
HCO3- conc blood plasma
A
27 mmol/l
21
Q
Panorg conc blood plasma
A
2 mmol/l
22
Q
fibrinogen conc blood plasma
A
3 (1-6) mmol/l
23
Q
glucose level ruminants blood plasma
A
3 (2-4) mmol/l
24
Q
glucose level monogastric animals blood plasma
A
5 (3-5.5) mmol/l
25
glucose level poultry blood plasma
10 (9-11) mmol/l
26
cholesterol level blood plasma
5 (1 - 8) mmol/l
27
residual nitrogen level blood plasma
15-25 mmol/l
28
urea blood plasma
3-10 mmol/l
29
creatinin blood plasma
50 - 200 umol/l
30
Hematocrit in venous blood average
0.35-0.45 l/l
35% - 45%
31
blood clotting time horse
20-25 min
32
blood clot time cattle
5-15min
33
blood clot time swine
5-6 min
34
blood clot time dog
6-7 min
35
blood clot time cat
7-9
36
Erythrocyte sedimentation rate horse
60- 90 mm/h
37
Erythrocyte sedimentation rate cattle
0-2 mm/h
38
Erythrocyte sedimentation rate swine
1-14mm/h
39
Erythrocyte sedimentation rate dog
5-10 mm/h
40
Erythrocyte sedimentation rate cat
5-10 mm/h
41
lymphocytic species: cattle, swine, sheep, domestic fowl
Lymphocyten
60 (55-75) %
42
lymphocytic species: cattle, swine, sheep, domestic fowl Neutrophil granulocyte
35 (15-45) %
43
lymphocytic species: cattle, swine, sheep, domestic fowl Eosinophil granulocyte
0-5
44
lymphocytic species: cattle, swine, sheep, domestic fowl basophil granulocyte
1
45
lymphocytic species: cattle, swine, sheep, domestic fowl Monocyte
1-10
46
neutrophilic species: horse, dog, cat Lymphocyte
35 (15-45)%
47
neutrophilic species: horse, dog, cat NEUTROPHIL GRANULOCYTE
60 (55-75) %
48
neutrophilic species: horse, dog, cat EOSINOPHIL GRANULOCYTE
0-8
49
neutrophilic species: horse, dog, cat BASOPHIL GRANULOCYTE
1
50
neutrophilic species: horse, dog, cat MONOCYTE
0-7 %
51
Protein conc of body fluids Mammals, blood plasma
60-80 g/l;
albumin 35-50 %, globulins 50-65 %
52
Protein conc of body fluids poultry, blood plasma
30-40 g/l
albumin 35-50 %, globulins 50-65 %
53
protein conc body fluids interstitial fluids
30-40g/l
54
protein conc of body fluid lymph
30-40g/l
55
protein conc of body fluids cerebrospinal fluid
0.25 - 0.4 g/l
56
Effects of heparin?
prevents blood from coagulating by catalysing the thrombin-antithrombin reaction
57
What is the function of methanol in fixation?
fix the smear and precipitate the proteins
58
Buffy coat, explain
White blood cells have lower density than red, and will form thin layer on the top after centrifugation
59
How to obtain blood serum
Centrifuge blood sample where fibrinogen is changed into fibrin by shaking in flask containing small glass beads
60
Defirbrinated blood
Shake flask with blood and small glass beads to change fibrinogen into fibrin
61
What happens with the hematocrit level while increasing the epinephrine level
Increase
62
Anticoagulants, give examples.
sodium citrate and Ethylene Diamine Tetraacetic Acid (EDTA)
63
Lee white method
Tilt blood in tube in intervals in 3 minutes to see if it coagulates
64
Def. of hematocrit
Ratio of red blood cells to the total blood volume
65
Venous hematocrit larger than arterial, explain.
Because of ion transport and water flux associated with the CO2 transport
66
Increased erythrocyte sedimentation rate
By displacing the albumin the surface charge decrease
67
By displacing the albumin the surface charge decrease
nucleus: beanshape (young), elongated (middleage), segmented (mature), light pink cytoplasm, different granules, 10 μm diameter, enzymes: lysozyme, myeloperoxidase)
68
Describe the hetrophil granulocytes (birds)
Nucleus: seveeral segments, pink cytoplasm, pale red granules
69
Describe the eosinophil granulocytes
Nucleus: segmented (usually bi-lobed), large bright red granules, 14 μm diameter, Enzmes: histaminase, acid & alkaine phosphatase, eosinphil basic & cationic protein)
70
Describe the basophil granulocytes
nucleus: hardly segmented, large blue granules, 15 μm diameter, Enzymes: protease
