PPP

Question 1

pentose phosphate pathway

Answer 1

once glucose-6-P is made, it will branch off from glycolysis and produce ribose-5-phosphate and NADPH

Question 2

PPP location

Answer 2

cytosol

Question 3

Where is there a high activity of the PPP?

Answer 3

erythrocytes, liver, adrenals, testes, ovary, mammary glands, and neutrophils/macrophages

Question 4

NADPH

Answer 4

used in fatty acid and steroid synthesis, by cytochrome P-450, regeneration of GSH, by neutrophils for phagolysosome activity, synthesis of NO, and nucleotide synthesis

Question 5

ribose-5-phosphate

Answer 5

used for nucleotide synthesis

Question 6

glutathione

Answer 6

detoxifies reactive oxygen species

Question 7

cytochrome p-450

Answer 7

catalyze reactions in steroid hormone synthesis and hydroxylation of non-polar drugs for excretion

Question 8

oxidative reactions of PPP

Answer 8

produce NADPH, irreversible

Question 9

non-oxidative reactions of PP

Answer 9

interconvert sugar phosphates, fully reversible

Question 10

first PPP oxidative reacton

Answer 10

oxidation of G6P into 6-phospho-gluconate by G6PDH; produces NADPH

Question 11

G6PDH

Answer 11

inhibited by NADPH

Question 12

second PPP oxidative reaction

Answer 12

6-phospho-gluconate into ribulose-5-phosphate by 6-phosphogluconate dehydrogenase

Question 13

non-oxidative reactions produce _

Answer 13

ultimately produce fructose-6-phosphate and glyceraldehyde-3-phosphate for formation of glucose-6-phosphate

Question 14

G6PDH deficiency

Answer 14

sensitivity to peroxides and free radicals resulting in cellular damage to RBCs, protein, and DNA; induces hemolytic anemia, jaundice, kidney failure

Question 15

location of mutation in G6PDH deficiency

Answer 15

if mutation is in coding region for NADP+ binding domain, the disease will be more severe

Question 16

hemolysis triggers in G6PDH deficiency

Answer 16

infections, treatments that require NADPH for metabolism (anti-malarials), and fava beans